Quality assurance considerations for detection of waterborne zoonotic parasites using Cryptosporidium oocyst detection as the main example

被引：2

作者：

Bellamy, JEC ^{[1
]}

机构：

[1] Univ Prince Edward Isl, Atlantic Vet Coll, Dept Pathol & Microbiol, Charlottetown, PE C1A 4P3, Canada

来源：

VETERINARY PARASITOLOGY | 2004年 / 126卷 / 1-2期

关键词：

quality assurance; detection methods; Cryptosporidium oocysts;

D O I：

10.1016/j.vetpar.2004.09.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A laboratory quality assurance (QA) program can minimize errors and provide confidence in the validity of laboratory test results. The structure of a QA program varies somewhat among laboratories but usually requires addressing a QA manual, QA goals, quality of resources, standard operating procedures, internal quality control, and external QA procedures. This paper reviews these general components and discusses some of the more particular QA considerations specific to filtration, immunomagnetic separation (IMS), immunofluorescence microscopy (FA), vital dye staining, differential interference contrast (DIC) microscopy, and molecular methods, which are involved in the detection and enumeration of Cryptosporidium oocysts. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：235 / 248

页数：14

共 24 条

[1]

[Anonymous], 1990, PCR PROTOCOLS GUIDE

[2]

BELLAMY JEC, 2000, QUALITY ASSURANCE HD

[3] EFFECT OF 3 CONCENTRATION TECHNIQUES ON VIABILITY OF CRYPTOSPORIDIUM-PARVUM OOCYSTS RECOVERED FROM BOVINE FECES [J].