Hydrolysis of cellulose using cellulase physically immobilized on highly stable zirconium based metal-organic frameworks

被引：91

作者：

Ahmed, Ibrahim Nasser ^{[1
,2
]}

Yang, Xiu-Li ^{[1
]}

Dubale, Amare Aregahegn ^{[1
,3
]}

Li, Ruo-Fei ^{[1
]}

Ma, Yi-Ming ^{[1
]}

Wang, Lu-Ming ^{[1
]}

Hou, Gui-Hua ^{[1
]}

Guan, Rong-Feng ^{[1
]}

Xie, Ming-Hua ^{[1
]}

机构：

[1] Yancheng Inst Technol, Key Lab Adv Technol Environm Protect Jiangsu Prov, Yancheng 224051, Peoples R China

[2] Addis Ababa Sci & Technol, Coll Appl Sci, Dept Ind Chem, POB 16417, Addis Ababa, Ethiopia

[3] Dilla Univ, Dept Chem, Coll Nat & Computat Sci, Energy & Environm Res Ctr, POB 419, Dilla, Ethiopia

来源：

BIORESOURCE TECHNOLOGY | 2018年 / 270卷

基金：

中国国家自然科学基金;

关键词：

Cellulase; Metal-organic frameworks; Immobilization; Hydrolysis; MULTIWALL CARBON NANOTUBES; COVALENT IMMOBILIZATION; ENZYME IMMOBILIZATION; STABILITY; MOF; CO2; SACCHARIFICATION; NANOPARTICLES; COMPOSITES; ADSORPTION;

D O I：

10.1016/j.biortech.2018.09.077

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Developing a new cellulase-MOF composite system with enhanced stability and reusability for cellulose hydrolysis was aimed. Physical adsorption strategy was employed to fabricate two cellulase composites, and the activity of composite was characterized by hydrolysis of carboxymethyl cellulose. The NH2 functionalized UiO-66-NH2 MOF exhibited higher protein loading than the precursor UiO-66, due to the extra anchor sites of NH2 groups. The immobilized cellulase showed enhanced thermostability, pH tolerance and lifetime. The maximum activity attained at 55 degrees C could be kept 85% when used at 80 degrees C, and the residual activities were 72% after ten cycles and 65% after 30 days storage. The abundant NH2 and COOH groups of MOF adsorb cellulase and enhance its stability, and the resulted heterogeneity offered the opportunity of recovering composite via mild centrifuge. The findings suggest the promising future of developing cellulase-MOF composite with ultrahigh activities and stabilities for practical application.

引用

页码：377 / 382

页数：6

共 43 条

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