Effect of vitamin E on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

被引:69
作者
Aras-Hisar, S [1 ]
Hisar, O
Beydemir, S
Gülcin, I
Yanik, T
机构
[1] Ataturk Univ, Fac Agr, Dept Aquaculture, TR-25240 Erzurum, Turkey
[2] Ataturk Univ, Arts & Sci Fac, Dept Chem, TR-25240 Erzurum, Turkey
关键词
carbonic anhydrase; erythrocyte; vitamin E; trout;
D O I
10.1556/AVet.52.2004.4.4
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Considering that the excessive usage of vitamin E causes hypervitaminosis and thus reduces blood erythrocyte concentrations, therefore it is worth studying how its pharmacological dosage affects the activity of carbonic anhydrase (CA) enzyme found in erythrocytes of rainbow trout (Oncorhynchus mykiss) in vitro and in vivo. Vitamin E inhibited CA enzyme and the IC50 value of the vitamin was 0.039 mM in vitro. Similarly, it was seen that vitamin E inhibited CA enzyme activity after the first hour following vitamin E injections in vivo. The activities of CA in groups of trout given vitamin E injection were measured at 1, 3 and 5 h and the corresponding activities were found to be 772.7 +/- 290.5 (P < 0.05), 1286.4 +/- 378.2 and 1005.7 +/- 436.1 enzyme units (EU) g Hb(-1). The difference over the control was significant (P < 0.05) in the first hour and insignificant at 3 and 5 h (P > 0.05). The activity of CA in the control, which did not contain vitamin E, was determined as 1597.7 +/- 429.0 EU g Hb(-1).
引用
收藏
页码:413 / 422
页数:10
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