Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein

被引:4
作者
Maruyama, Nobuyuki [1 ]
Goshi, Tomohiro [1 ]
Sugiyama, Shigeru [2 ]
Niiyama, Mayumi [3 ]
Adachi, Hiroaki [4 ]
Takano, Kazufumi [4 ,5 ]
Murakami, Satoshi [4 ,6 ]
Inoue, Tsuyoshi [3 ,4 ]
Mori, Yusuke [3 ,4 ]
Matsumura, Hiroyoshi [3 ,4 ]
Mikami, Bunzo [7 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Lab Food Qual Design & Dev, Kyoto 6110011, Japan
[2] Osaka Univ, Grad Sch Sci, Toyonaka, Osaka 5608531, Japan
[3] Osaka Univ, Grad Sch Engn, Suita, Osaka 5650871, Japan
[4] SOSHO Inc, Suita, Osaka 5650871, Japan
[5] Kyoto Prefectural Univ, Grad Sch Life & Environm Sci, Sakyo Ku, Kyoto 6068522, Japan
[6] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan
[7] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Lab Appl Struct Biol, Kyoto 6110011, Japan
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2015年 / 71卷
关键词
soybean; seed storage protein; beta-conglycinin; vacuolar sorting receptor; C-TERMINAL SEQUENCE; BETA-CONGLYCININ; LIGAND-BINDING; FEATURES; CELLS;
D O I
10.1107/S2053230X14027484
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
beta-Conglycinin is a major seed storage protein in soybeans, which are an important source of protein. The major subunits (alpha, alpha' and beta) of beta-conglycinin are sorted to protein-storage vacuoles in seed cells. Vacuolar sorting receptor (VSR) is an integral membrane protein that recognizes the sorting determinant of vacuolar proteins, including beta-conglycinin, and regulates their sorting process. Vacuolar sorting determinants of the alpha' and beta subunits of beta-conglycinin exist in their C-terminal peptides. Here, the preliminary X-ray diffraction analysis of the binding domain of soybean VSR crystallized with the peptide responsible for the sorting determinant in beta-conglycinin is reported. X-ray diffraction data were collected to a resolution of 3.5 angstrom. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 116.4, c = 86.1 angstrom.
引用
收藏
页码:132 / 135
页数:4
相关论文
共 15 条
[1]   Cloning and subcellular location of an Arabidopsis receptor-like protein that shares common features with protein-sorting receptors of eukaryotic cells [J].
Ahmed, SU ;
BarPeled, M ;
Raikhel, NV .
PLANT PHYSIOLOGY, 1997, 114 (01) :325-336
[2]   Structural requirements for ligand binding by a probable plant vacuolar sorting receptor [J].
Cao, XF ;
Rogers, SW ;
Butler, J ;
Beevers, L ;
Rogers, JC .
PLANT CELL, 2000, 12 (04) :493-506
[3]   PURIFICATION AND INITIAL CHARACTERIZATION OF A POTENTIAL PLANT VACUOLAR TARGETING RECEPTOR [J].
KIRSCH, T ;
PARIS, N ;
BUTLER, JM ;
BEEVERS, L ;
ROGERS, JC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3403-3407
[4]   The roles of the N-linked glycans and extension regions of soybean β-conglycinin in folding, assembly and structural features [J].
Maruyama, N ;
Katsube, T ;
Wada, Y ;
Oh, MH ;
De la Rosa, APB ;
Okuda, E ;
Nakagawa, S ;
Utsumi, S .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 258 (02) :854-862
[5]   The composition of newly synthesized proteins in the endoplasmic reticulum determines the transport pathways of soybean seed storage proteins [J].
Mori, T ;
Maruyama, N ;
Nishizawa, K ;
Higasa, T ;
Yagasaki, K ;
Ishimoto, M ;
Utsumi, S .
PLANT JOURNAL, 2004, 40 (02) :238-249
[6]   Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein [J].
Morimoto, Shota ;
Tomohiro, Takenori ;
Maruyama, Nobuyuki ;
Hatanaka, Yasumaru .
CHEMICAL COMMUNICATIONS, 2013, 49 (18) :1811-1813
[7]   A vacuolar sorting determinant of soybean β-conglycinin β subunit resides in a C-terminal sequence [J].
Nishizawa, K ;
Maruyama, N ;
Satoh, R ;
Higasa, T ;
Utsumi, S .
PLANT SCIENCE, 2004, 167 (04) :937-947
[8]   A C-terminal sequence of soybean β-conglycinin α′ subunit acts as a vacuolar sorting determinant in seed cells [J].
Nishizawa, K ;
Maruyama, N ;
Satoh, R ;
Fuchikami, Y ;
Higasa, T ;
Utsumi, S .
PLANT JOURNAL, 2003, 34 (05) :647-659
[9]   Processing of X-ray diffraction data collected in oscillation mode [J].
Otwinowski, Z ;
Minor, W .
MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326
[10]   Structural characterization of the N-glycosylation of individual soybean β-conglycinin subunits [J].
Picariello, Gianluca ;
Amigo-Benavent, Miryam ;
del Castillo, Maria Dolores ;
Ferranti, Pasquale .
JOURNAL OF CHROMATOGRAPHY A, 2013, 1313 :96-102