Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

被引：63

作者：

Liu, Ningning ^{[1
]}

Peng, Bo ^{[2
]}

Lin, Yuan ^{[2
,3
,4
]}

Su, Zhaohui ^{[2
]}

Niu, Zhongwei ^{[3
,4
]}

Wang, Qian ^{[3
,4
]}

Zhang, Wenke ^{[1
]}

Li, Hongbin ^{[5
]}

Shen, Jiacong ^{[1
]}

机构：

[1] Jilin Univ, State Key Lab Supramol Struct & Mat, Coll Chem, Changchun 130012, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China

[3] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA

[4] Univ S Carolina, NanoCtr, Columbia, SC 29208 USA

[5] Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 32期

关键词：

DNA-MOLECULES; RESOLUTION; AFM; MICROSCOPY; PROTEIN; TITIN; MECHANOCHEMISTRY; PARTICLE; EVENTS;

D O I：

10.1021/ja1052544

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.

引用

页码：11036 / 11038

页数：3

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