Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics

被引：40

作者：

Mertens, Haydyn D. T. ^{[1
]}

Svergun, Dmitri I. ^{[1
]}

机构：

[1] EMBL Hamburg DESY, Bldg 25A,Notkestr 85, D-22603 Hamburg, Germany

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2017年 / 628卷

关键词：

SAXS; NMR; Proteins; Structure; Dynamics; RESIDUAL DIPOLAR COUPLINGS; BIOLOGICAL MACROMOLECULES; WEB SERVER; PROTEIN STRUCTURES; ESCHERICHIA-COLI; JOSEPHIN DOMAIN; PROGRAM PACKAGE; XPLOR-NIH; SAXS; RESOLUTION;

D O I：

10.1016/j.abb.2017.05.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small-angle X-ray scattering (SAXS) and Nuclear Magnetic Resonance (NMR) are established methods to analyze the structure and structural transitions of biological macromolecules in solution. Both methods are directly applicable to near-native macromolecular solutions and allow one to study structural responses to physical and chemical changes or ligand additions. Whereas SAXS is applied to elucidate overall structure, interactions and flexibility over a wide range of particle sizes, NMR yields atomic resolution detail for moderately sized macromolecules. NMR is arguably the most powerful technique for the experimental analysis of dynamics. The joint application of these two highly complementary techniques provides an extremely useful approach that facilitates comprehensive characterization of bio-macromolecular solutions. (C) 2017 The Authors. Published by Elsevier Inc.

引用

页码：33 / 41

页数：9

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