Elucidation of cell secretion: Pancreas led the way

被引:18
作者
Craciun, C [1 ]
机构
[1] Univ Babes Bolyai, Dept Biol, Ctr Electron Microscopy, RO-400006 Cluj Napoca, Romania
关键词
porosomes; fusion pores; SNARE-induced membrane fusion; SNAREs; cell secretion; molecular level;
D O I
10.1159/000080230
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Secretion is a basic process in all cells and is required for several important functions such as neurotransmission, the secretion of digestive juices from the exocrine pancreas and the release of hormones from endocrine and neuroendocrine cells. Due to these important functions, the mechanism of cell secretion has been intensely investigated for over half a century. However, it is only in the last decade, with the discovery of a new cellular structure, the 'porosome' or 'fusion pore', and the elucidation of SNARE-induced membrane fusion, that has finally provided us with an understanding of cell secretion at the molecular level. The 'porosome', a supramolecular structure at the cell plasma membrane, was first discovered in the exocrine pancreas, and subsequently in endocrine/neuroendocrine cells and in neurons. The structure and dynamics of the 'porosome' in live cells at nanometer resolution and in real-time, its composition and functional reconstitution in lipid membrane, have all been determined. These findings have fundamentally changed our understanding of cell secretion and provide a clear understanding of this highly regulated process in cells. Copyright (C) 2004 S. Karger AG, Basel and IAP.
引用
收藏
页码:487 / 489
页数:3
相关论文
共 32 条
[1]   Regulation of the water channel aquaporin-1: isolation and reconstitution of the regulatory complex [J].
Abu-Hamdah, R ;
Cho, WJ ;
Cho, SJ ;
Jeremic, A ;
Kelly, M ;
Ilie, AE ;
Jena, BP .
CELL BIOLOGY INTERNATIONAL, 2004, 28 (01) :7-17
[2]   Single synaptic vesicles fusing transiently and successively without loss of identity [J].
Aravanis, AM ;
Pyle, JL ;
Tsien, RW .
NATURE, 2003, 423 (6940) :643-647
[3]   DEPLETION OF VESICLES FROM FROG NEUROMUSCULAR JUNCTIONS BY PROLONGED TETANIC STIMULATION [J].
CECCARELLI, B ;
HURLBUT, WP ;
MAURO, A .
JOURNAL OF CELL BIOLOGY, 1972, 54 (01) :30-+
[4]   ARREST OF MEMBRANE-FUSION EVENTS IN MAST-CELLS BY QUICK-FREEZING [J].
CHANDLER, DE ;
HEUSER, JE .
JOURNAL OF CELL BIOLOGY, 1980, 86 (02) :666-674
[5]   Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles [J].
Cho, SJ ;
Sattar, AKMA ;
Jeong, EH ;
Satchi, M ;
Cho, JA ;
Dash, S ;
Mayes, MS ;
Stromer, MH ;
Jena, BP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (07) :4720-4724
[6]   Structure and dynamics of the fusion pores in live GH-secreting cells revealed using atomic force microscopy [J].
Cho, SJ ;
Jeftinija, K ;
Glavaski, A ;
Jeftinija, S ;
Jena, BP ;
Anderson, LL .
ENDOCRINOLOGY, 2002, 143 (03) :1144-1148
[7]   New structure involved in transient membrane fusion and exocytosis [J].
Cho, SJ ;
Wakade, A ;
Pappas, GD ;
Jena, BP .
CHROMAFFIN CELL: TRNSMITTER BIOSYNTHESIS, STORAGE, RELEASE, ACTIONS, AND INFORMATICS, 2002, 971 :254-256
[8]   SNARES in opposing bilayers interact in a circular array to form conducting pores [J].
Cho, SJ ;
Kelly, M ;
Rognlien, KT ;
Cho, JA ;
Hörber, JKH ;
Jena, BP .
BIOPHYSICAL JOURNAL, 2002, 83 (05) :2522-2527
[9]   Structure and dynamics of the fusion pore in live cells [J].
Cho, SJ ;
Quinn, AS ;
Stromer, MH ;
Dash, S ;
Cho, J ;
Taatjes, DJ ;
Jena, BP .
CELL BIOLOGY INTERNATIONAL, 2002, 26 (01) :35-42
[10]   SNAPS, A FAMILY OF NSF ATTACHMENT PROTEINS INVOLVED IN INTRACELLULAR MEMBRANE-FUSION IN ANIMALS AND YEAST [J].
CLARY, DO ;
GRIFF, IC ;
ROTHMAN, JE .
CELL, 1990, 61 (04) :709-721