Ethanol inhibition of adenosine 5′-triphosphate-activated current in freshly isolated adult rat hippocampal CA1 neurons

被引：24

作者：

Li, CY ^{[1
]}

Xiong, KM ^{[1
]}

Weight, FF ^{[1
]}

机构：

[1] NIAAA, Mol & Cellular Neurobiol Lab, NIH, Bethesda, MD 20892 USA

来源：

NEUROSCIENCE LETTERS | 2000年 / 295卷 / 03期

关键词：

adenosine 5 '-triphosphate; ion channels; P2X receptors; hippocampus; alcohol;

D O I：

10.1016/S0304-3940(00)01586-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effect of ethanol on current activated by extracellular adenosine 5'-triphosphate (ATP) was studied in freshly isolated adult rat hippocampal CA1 neurons using whole-cell patch-clamp recording. ATP activated an inward current with an EC50 value of 18 muM. The inward current was also activated by 2-methylthio ATP (2-MeSATP) and cr,P-methylene ATP (alpha,beta -MeATP), inhibited by pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), and potentiated by Zn2+, Ethanol inhibited current activated by 10 muM ATP with an IC50 value of 83 mM in a voltage-independent manner; Ethanol, 100 mM, shifted the ATP concentration-response curve to the right, increasing the EC50 value for ATP from 18 to 33 muM, but did not reduce the maximal response to ATP. The results suggest that ethanol can inhibit the function of P2X receptors in adult rat hippocampal neurons by decreasing the apparent affinity of the binding site for ATP, (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：77 / 80

页数：4

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