Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste ("alperujo") composting

被引：16

作者：

Tortosa, German ^{[1
]}

Torralbo, Fernando ^{[2
]}

Maza-Marquez, Paula ^{[3
,4
,5
]}

Aranda, Elisabet ^{[3
,4
]}

Calvo, Concepcion ^{[3
,4
]}

Gonzalez-Murua, Carmen ^{[2
]}

Bedmar, Eulogio J. ^{[1
]}

机构：

[1] CSIC, Agencia Estatal, EEZ, Dept Soil Microbiol & Symbiot Syst, C Prof Albareda 1, E-18008 Granada, Spain

[2] Univ Basque Country, UPV EHU, Dept Plant Biol & Ecol, Barrio Sarriena S-N, Leioa 48940, Bizkaia, Spain

[3] Univ Granada, Inst Water Res, Edificio Fray Luis,C Ramon y Cajal 4, E-18071 Granada, Spain

[4] Univ Granada, Fac Pharm, Dept Microbiol, Campus Cartuja S-N, E-18071 Granada, Spain

[5] NASA, Exobiol Branch, Ames Res Ctr, Moffett Field, CA USA

来源：

BIORESOURCE TECHNOLOGY | 2020年 / 295卷

关键词：

Fungal diversity; Illumina MiSeq; Two-phase olive mill waste; qPCR; cDNA; MICROBIAL COMMUNITY; MANURE; FERTILIZERS; SUCCESSION; STRATEGIES; EVOLUTION; DYNAMICS;

D O I：

10.1016/j.biortech.2019.122267

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Metagenomic and transcriptomic techniques applied to composting could increase our understanding of the overall microbial ecology and could help us to optimise operational conditions which are directly related with economic interest. In this study, the fungal diversity and abundance of two-phase olive mill waste ("alperujo") composting was studied using Illumina MiSeq sequencing and quantitative PCR, respectively. The results showed an increase of the fungal diversity during the process, with Ascomycota being the predominant phylum. Penicillium was the main genera identified at the mesophilic and maturation phases, with Debaryomyces and Sarocladium at the thermophilic phase, respectively. The fungal abundance was increased during composting, which confirms their important role during thermophilic and maturation phases. Some Basidiomycota showed an increased during the process, which showed a positive correlation with the humification parameters. According to that, the genus Cystofilobasidium could be used as a potential fungal biomarker to assess alperujo compost maturation.

引用

页数：9

共 40 条

[1] Agrochemical characterisation of "alperujo", a solid by-product of the two-phase centrifugation method for olive oil extraction [J].