Detection of Free-Living Amoebae Using Amoebal Enrichment in a Wastewater Treatment Plant of Gauteng Province, South Africa

被引:17
作者
Muchesa, P. [1 ]
Mwamba, O. [1 ]
Barnard, T. G. [1 ]
Bartie, C. [1 ,2 ]
机构
[1] Univ Johannesburg, Water & Hlth Res Ctr, ZA-2028 Doornfontein, South Africa
[2] Natl Inst Occupat Hlth, ZA-2000 Johannesburg, South Africa
关键词
ACANTHAMOEBA-CASTELLANII; RESISTING BACTERIA; DRINKING-WATER; THERMOTOLERANT; IDENTIFICATION; CULTIVATION; PATHOGENS; STRAINS; AGENTS; HEAT;
D O I
10.1155/2014/575297
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Free-living amoebae pose a potential health risk in water systems as they may be pathogenic and harbor potential pathogenic bacteria known as amoebae resistant bacteria. Free-living amoebae were observed in 150 (87.2%) of the environmental water samples. In particular, Acanthamoeba sp. was identified in 22 (12.8%) using amoebal enrichment and confirmed by molecular analysis. FLA were isolated in all 8 stages of the wastewater treatment plant using the amoebal enrichment technique. A total of 16 (9.3%) samples were positive for FLA from influent, 20 (11.6%) from bioreactor feed, 16 (9.3%) from anaerobic zone, 16 (9.3%) from anoxic zone, 32 (18.6%) from aerators, 16 (9.3%) from bioreactor effluent, 11 (6.4%) from bioreactor final effluent, and 45 (26.2%) from maturation pond. This study provides baseline information on the occurrence of amoebae in wastewater treatment plant. This has health implications on receiving water bodies as some FLA are pathogenic and are also involved in the transmission and dissemination of pathogenic bacteria.
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页数:10
相关论文
共 37 条
[1]   Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii [J].
Abd, Hadi ;
Saeed, Amir ;
Weintraub, Andrej ;
Nair, G. Balakrish ;
Sandstrom, Gunnar .
FEMS MICROBIOLOGY ECOLOGY, 2007, 60 (01) :33-39
[2]  
BOSE K, 1990, BRAZ J MED BIOL RES, V23, P1271
[3]   Novel Chlamydiales strains isolated from a water treatment plant [J].
Corsaro, Daniele ;
Feroldi, Veronica ;
Saucedo, Gemma ;
Ribas, Ferran ;
Loret, Jean-Francois ;
Greub, Gilbert .
ENVIRONMENTAL MICROBIOLOGY, 2009, 11 (01) :188-200
[4]  
da Rocha-Azevedo B, 2009, INTERDISCIP PERSPECT, V2009, DOI DOI 10.1155/2009/251406
[5]   Distribution of free-living amoebae in James River, Virginia, USA [J].
Ettinger, MR ;
Webb, SR ;
Harris, SA ;
McIninch, SP ;
Garman, GC ;
Brown, BL .
PARASITOLOGY RESEARCH, 2003, 89 (01) :6-15
[6]   Potentially pathogenic free-living amoebae (FLA) isolated in Spanish wastewater treatment plants [J].
Garcia, A. ;
Goni, P. ;
Clavel, A. ;
Lobez, S. ;
Fernandez, M. T. ;
Ormad, M. P. .
ENVIRONMENTAL MICROBIOLOGY REPORTS, 2011, 3 (05) :622-626
[7]   Identification of Free-Living Amoebae and Amoeba-Associated Bacteria from Reservoirs and Water Treatment Plants by Molecular Techniques [J].
Garcia, Alicia ;
Goni, Pilar ;
Cieloszyk, Joanna ;
Teresa Fernandez, Maria ;
Calvo-Begueria, Laura ;
Rubio, Encarnacion ;
Francisca Fillat, Maria ;
Luisa Peleato, Maria ;
Clavel, Antonio .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2013, 47 (07) :3132-3140
[8]   Identifying endosymbiont bacteria associated with free-living amoebae [J].
Goni, Pilar ;
Teresa Fernandez, Maria ;
Rubio, Encarnacion .
ENVIRONMENTAL MICROBIOLOGY, 2014, 16 (02) :339-349
[9]   Microorganisms resistant to free-living amoebae [J].
Greub, G ;
Raoult, D .
CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (02) :413-+
[10]  
Health Protection Agency, 2003, W17I22 CDSC STAND UN