Selective inhibition of Alu retrotransposition by APOBEC3G

被引：116
作者：
Hulme, Amy E. [1 ]
Bogerd, Hal P.
Cullen, Bryan R.
Moran, John V.
机构：
[1] Univ Michigan, Sch Med, Dept Human Genet, Ann Arbor, MI 48109 USA
[2] Duke Univ, Med Ctr, Ctr Virol, Durham, NC 27710 USA
[3] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA
[4] Univ Michigan, Sch Med, Dept Internal Med, Ann Arbor, MI 48109 USA
来源：
GENE | 2007年 / 390卷 / 1-2期
关键词：
LINE-1; retrotransposon; APOBEC; transposable element;
D O I：
10.1016/j.gene.2006.08.032
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The non-LTR retrotransposon LINE-1 (L1) comprises similar to 17% of the human genome, and the L1-encoded proteins can function in trans to mediate the retrotransposition of non-autonomous retrotransposons (i.e., Alu and probably SVA elements) and cellular mRNAs to generate processed pseudogenes. Here, we have examined the effect of APOBEC3G and APOBEC3F, cytidine deaminases that inhibit Vif-deficient HIV-1 replication, on Alu retrotransposition and other L1-mediated retrotransposition processes. We demonstrate that APOBEC3G selectively inhibits Alu retrotransposition in an OR-F I p-in dependent manner. An active cytidine deaminase site is not required for the inhibition of Alu retrotransposition and the resultant integration events lack G to A or C to T hypermutation. These data demonstrate a differential restriction of L1 and Alu retrotransposition by APOBEC3G, and suggest that the Alu ribonucleoprotein complex may be targeted by APOBEC3G. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：199 / 205
页数：7
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