BIOCHEMISTRY The inner workings of an enzyme

被引:2
作者
Baumer, Zachary T. [1 ]
Whitehead, Timothy A. [1 ]
机构
[1] Univ Colorado, Dept Chem & Biol Engn, Boulder, CO 80305 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
D O I
10.1126/science.abj8346
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
引用
收藏
页码:391 / +
页数:2
相关论文
共 10 条
[1]   Deep mutational scanning: assessing protein function on a massive scale [J].
Araya, Carlos L. ;
Fowler, Douglas M. .
TRENDS IN BIOTECHNOLOGY, 2011, 29 (09) :435-442
[2]   The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters [J].
Bar-Even, Arren ;
Noor, Elad ;
Savir, Yonatan ;
Liebermeister, Wolfram ;
Davidi, Dan ;
Tawfik, Dan S. ;
Milo, Ron .
BIOCHEMISTRY, 2011, 50 (21) :4402-4410
[3]   DIRECT, REAL-TIME MEASUREMENT OF RAPID INORGANIC-PHOSPHATE RELEASE USING A NOVEL FLUORESCENT-PROBE AND ITS APPLICATION TO ACTOMYOSIN SUBFRAGMENT-1 ATPASE [J].
BRUNE, M ;
HUNTER, JL ;
CORRIE, JET ;
WEBB, MR .
BIOCHEMISTRY, 1994, 33 (27) :8262-8271
[4]   A Comprehensive, High-Resolution Map of a Gene's Fitness Landscape [J].
Firnberg, Elad ;
Labonte, Jason W. ;
Gray, Jeffrey J. ;
Ostermeier, Marc .
MOLECULAR BIOLOGY AND EVOLUTION, 2014, 31 (06) :1581-1592
[5]   Principles of Protein Stability and Their Application in Computational Design [J].
Goldenzweig, Adi ;
Fleishman, Sarel J. .
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 87, 2018, 87 :105-129
[6]   Design of Protein Catalysts [J].
Hilvert, Donald .
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 82, 2013, 82 :447-470
[7]   Using deep mutational scanning to benchmark variant effect predictors and identify disease mutations [J].
Livesey, Benjamin J. ;
Marsh, Joseph A. .
MOLECULAR SYSTEMS BIOLOGY, 2020, 16 (07)
[8]   Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics [J].
Markin, C. J. ;
Mokhtari, D. A. ;
Sunden, F. ;
Appel, M. J. ;
Akiva, E. ;
Longwell, S. A. ;
Sabatti, C. ;
Herschlag, D. ;
Fordyce, P. M. .
SCIENCE, 2021, 373 (6553) :411-+
[9]   An Automated Data-Driven Pipeline for Improving Heterologous Enzyme Expression [J].
Wrenbeck, Emily E. ;
Bedewitz, Matthew A. ;
Klesmith, Justin R. ;
Noshin, Syeda ;
Barry, Cornelius S. ;
Whitehead, Timothy A. .
ACS SYNTHETIC BIOLOGY, 2019, 8 (03) :474-481
[10]   Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded [J].
Wrenbeck, Emily E. ;
Azouz, Laura R. ;
Whitehead, Timothy A. .
NATURE COMMUNICATIONS, 2017, 8