Study on lipid peroxidation potential in different tissues induced by ascorbate-Fe2+:: Possible factors involved in their differential susceptibility

被引：13

作者：

Pushpendran, CK ^{[1
]}

Subramanian, M ^{[1
]}

Devasagayam, TPA ^{[1
]}

Singh, BB ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Radiat Biol & Biochem Div, Mumbai 400085, India

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1998年 / 178卷 / 1-2期

关键词：

lipid peroxidation; ascorbate-Fe2+; thiobarbituric acid reactive substances; malonaldehyde equivalents; oxidative stress; antioxidants;

D O I：

10.1023/A:1006895531504

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Susceptibility of four major rat tissues to oxidative damage in terms of lipid peroxidation induced by in vitro by ascorbate-Fe2+ in homogenates and mitochondria has been examined. Lipid peroxidation, as assessed by thiobarbituric acid reactive substances (TEARS) and conjugated dienes was maximum in brain followed by liver, kidney and heart. However, the time course of lipid peroxidation showed different patterns in tissues examined. The higher susceptibilities of brain and liver can be explained by substrate availability and to a lesser extent the level of antioxidants. The differences observed in the tissues studied may reflect their susceptibility to degenerative diseases and xenobiotic toxicity which are considered as a result of oxidative damage to membranes.

引用

页码：197 / 202

页数：6

共 44 条

[1] APPLICATION OF THE THIOBARBITURATE ASSAY TO THE MEASUREMENT OF LIPID-PEROXIDATION PRODUCTS IN MICROSOMES [J].

ALBRO, PW ;

CORBETT, JT ;

SCHROEDER, JL .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1986, 13 (03) :185-194

[2]

BAKER N, 1966, J LIPID RES, V7, P349

[3] A DECREASE OF FREE-RADICAL PRODUCTION NEAR-CRITICAL TARGETS AS A CAUSE OF MAXIMUM LONGEVITY IN ANIMALS [J].

BARJA, G ;

CADENAS, S ;

ROJAS, C ;

LOPEZTORRES, M ;

PEREZCAMPO, R .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1994, 108 (04) :501-512

[4]

BEST CH, 1980, LIVING BODY, P21

[5]

Buege J A, 1978, Methods Enzymol, V52, P302

[6] THE PECKING ORDER OF FREE-RADICALS AND ANTIOXIDANTS - LIPID-PEROXIDATION, ALPHA-TOCOPHEROL, AND ASCORBATE [J].

BUETTNER, GR .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (02) :535-543

[7]

BURTON GW, 1986, ACCOUNTS CHEM RES, V19, P194, DOI 10.1021/ar00127a001

[8]

Bus J. S., 1979, REV BIOCHEM TOXICOL, V1, P125

[9] PEROXIDE-PRODUCING POTENTIAL OF TISSUES - INVERSE CORRELATION WITH LONGEVITY OF MAMMALIAN-SPECIES [J].

CUTLER, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (14) :4798-4802

[10]

DESAI ID, 1984, METHOD ENZYMOL, V105, P1838

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