Drought and UV Radiation Stress Tolerance in Rice Is Improved by Overaccumulation of Non-Enzymatic Antioxidant Flavonoids

被引：68

作者：

Jan, Rahmatullah ^{[1
,2
]}

Khan, Muhammad-Aaqil ^{[1
]}

Asaf, Sajjad ^{[3
]}

Lubna ^{[4
]}

Waqas, Muhammad ^{[5
]}

Park, Jae-Ryoung ^{[1
,6
]}

Asif, Saleem ^{[1
]}

Kim, Nan ^{[1
]}

Lee, In-Jung ^{[1
]}

Kim, Kyung-Min ^{[1
,2
]}

机构：

[1] Kyungpook Natl Univ, Coll Agr & Life Sci, Dept Appl Biosci, Div Plant Biosci, 80 Dahak Ro, Daegu 41566, South Korea

[2] Kyungpook Natl Univ, Costal Agr Res Inst, 80 Dahak Ro, Daegu 41566, South Korea

[3] Univ Nizwa, Nat & Med Sci Res Ctr, Nizwa 616, Oman

[4] Abdul Wali Khan Univ, Dept Bot, Garden Campus, Mardan 23200, Pakistan

[5] Govt Khyber Pakhtunkhwa, Dept Agr Extens, Mardan 23200, Pakistan

[6] Rural Dev Adm, Crop Breeding Div, Natl Inst Crop Sci, Wonju 55365, South Korea

来源：

ANTIOXIDANTS | 2022年 / 11卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

drought and UV radiation stress; flavonoids content; antioxidant content; salicylic acid; genes expression; ULTRAVIOLET-B RADIATION; SIGNALING COMPONENT UVR8; GENOME-WIDE ANALYSIS; FLAVANONE; 3-HYDROXYLASE; GENE-EXPRESSION; SALICYLIC-ACID; DEHYDRIN GENE; ARABIDOPSIS; PLANT; BIOSYNTHESIS;

D O I：

10.3390/antiox11050917

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drought and ultraviolet radiation (UV radiation) are the coexisting environmental factors that negatively affect plant growth and development via oxidative damage. Flavonoids are reactive, scavenging oxygen species (ROS) and UV radiation-absorbing compounds generated under stress conditions. We investigated the biosynthesis of kaempferol and quercetin in wild and flavanone 3-hydroxylase (F3H) overexpresser rice plants when drought and UV radiation stress were imposed individually and together. Phenotypic variation indicated that both kinds of stress highly reduced rice plant growth parameters in wild plants as compared to transgenic plants. When combined, the stressors adversely affected rice plant growth parameters more than when they were imposed individually. Overaccumulation of kaempferol and quercetin in transgenic plants demonstrated that both flavonoids were crucial for enhanced tolerance to such stresses. Oxidative activity assays showed that kaempferol and quercetin overaccumulation with strong non-enzymatic antioxidant activity mitigated the accumulation of ROS under drought and UV radiation stress. Lower contents of salicylic acid (SA) in transgenic plants indicated that flavonoid accumulation reduced stress, which led to the accumulation of low levels of SA. Transcriptional regulation of the dehydrin (DHN) and ultraviolet-B resistance 8 (UVR8) genes showed significant increases in transgenic plants compared to wild plants under stress. Taken together, these results confirm the usefulness of kaempferol and quercetin in enhancing tolerance to both drought and UV radiation stress.

引用

页数：20

共 94 条

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