Genomic detection and molecular characterization of two distinct isolates of cycas necrotic stunt virus from Paeonia suffruticosa and Daphne odora

被引:7
作者
Lim, Seungmo [1 ]
Kwon, Suk-Yoon [1 ]
Lee, Joong-Hwan [2 ]
Cho, Hye Sun [1 ]
Kim, Hyun-Soon [1 ]
Park, Jeong Mee [1 ]
Lee, Su-Heon [3 ]
Moon, Jae Sun [1 ]
机构
[1] Korea Res Inst Biosci & Biotechnol, Plant Syst Engn Res Ctr, Daejeon 34141, South Korea
[2] Inst Bioresources Res, Gyeongsangbuk Do Agr Res & Extens Serv, Andong 36614, South Korea
[3] Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Daegu 41566, South Korea
关键词
Cycas necrotic stunt virus; Nepovirus; Paeonia suffruticosa; Daphne odora; HTS; TAXONOMY; SEQUENCE;
D O I
10.1007/s11262-019-01687-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Complete genome sequences of two cycas necrotic stunt virus (CNSV) isolates from Paeonia suffruticosa and Daphne odora were determined. Phylogenetic trees and pairwise comparisons using complete RNA1- and RNA2-encoded polyproteins showed that the two CNSV isolates are divergent (83.19%-89.42% in polyprotein 1 and 73.61%-85.78% in polyprotein 2). A comparative analysis based on taxonomic criteria for the species demarcation of nepoviruses confirmed that they are not new species but distinct variants. This is the first report of the complete genome sequences of CNSV detected in P. suffruticosa and D. odora, and the first report of CNSV infecting P. suffruticosa.
引用
收藏
页码:734 / 737
页数:4
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