Evaluation of biodegradation and biocompatibility of collagen/chitosan/alkaline phosphatase biopolymeric membranes

被引：6

作者：

Berteanu, E. ^{[1
]}

Ionita, D. ^{[2
]}

Simoiu, M. ^{[3
]}

Paraschiv, M. ^{[1
]}

Tatia, R. ^{[1
]}

Apatean, A. ^{[1
]}

Sidoroff, M. ^{[1
]}

Tcacenco, L. ^{[1
]}

机构：

[1] Natl Inst Res & Dev Biol Sci, Bucharest 060031, Romania

[2] Politehn Bucharest Univ, Fac Appl Chem & Mat Sci, Dept Gen Chem, Bucharest 011061, Romania

[3] Natl Inst Infect Dis Prof Dr Matei Bals, Bucharest 021105, Romania

来源：

BULLETIN OF MATERIALS SCIENCE | 2016年 / 39卷 / 02期

关键词：

Collagen; chitosan; biocompatibility; adsorption; COLORIMETRIC ASSAY; IN-VITRO; CHITOSAN; SUBSTRATE; HYDROGEL; FILMS; VIVO;

D O I：

10.1007/s12034-016-1161-7

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The aim of this study was to develop a new variant of membranes based on collagen (COL), chitosan (CHI) and alkaline phosphatase (ALP) immobilized and cross-linking with glutaraldehyde (GA) at different concentrations. The biodegradation in the presence of collagenase was investigated. Biocompatibility was evaluated by MTT assay using a mouse fibroblast cell culture type NCTC (clone 929). Non-cross-linked samples were biocompatible and membranes cross-linked with low concentrations of GA (0.04, 0.08%) were also biocompatible. However, high concentrations of GA lead to a decreased biocompatibility. The adsorption behaviour of Ca2+ ions to all membranes were evaluated using the Freundlich isotherms. Haemolytic studies were performed in order to consider their applications in biomineralization process. By the addition of collagen and ALP to chitosan, the haemolytic index decreases, the COL-CHI-ALP membrane being in the non-haemolytic domain, while the COL-CHI-ALP-GA membrane has a haemolytic index greater than 2, and is slightly haemolytic.

引用

页码：377 / 383

页数：7

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