Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil

被引：42

作者：

Prichula, Janira ^{[1
,2
]}

Pereira, Rebeca Inhoque ^{[1
,2
]}

Wachholz, Guilherme Raffo ^{[2
]}

Cardoso, Leonardo Almansa ^{[1
]}

Correa Tolfo, Neidimar Cezar ^{[2
]}

Santestevan, Naiara Aguiar ^{[1
]}

Medeiros, Aline Weber ^{[1
]}

Tavares, Mauricio ^{[3
]}

Frazzon, Jeverson ^{[4
]}

d'Azevedo, Pedro Alves ^{[2
]}

Guedes Frazzon, Ana Paula ^{[1
]}

机构：

[1] Fed Univ Rio Grande Sul UFRGS, Dept Microbiol Immunol & Parasitol, Sarmento Leite 500,Room 158, BR-90050170 Porto Alegre, RS, Brazil

[2] Fed Univ Hlth Sci Porto Alegre UFCSPA, Gram Posit Coccus Lab, Sarmento Leite 245,Room 204, BR-90050170 Porto Alegre, RS, Brazil

[3] UFRGS, Inst Biosci, Ctr Coastal Studies Limnol & Marine CECLIMAR, Av Tramandai 976, BR-95625000 Imbe, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Inst Food Sci, Av Bento Goncalves 9500, BR-91501970 Porto Alegre, RS, Brazil

来源：

MARINE POLLUTION BULLETIN | 2016年 / 105卷 / 01期

关键词：

Wild marine species; Enterococci resistant; Resistance genes; SEALS PHOCA-VITULINA; ANTIBIOTIC-RESISTANCE; MOLECULAR CHARACTERIZATION; AQUATIC ENVIRONMENT; MAGELLANIC PENGUINS; ESCHERICHIA-COLI; GENE TET(M); PCR ASSAY; IDENTIFICATION; BACTERIA;

D O I：

10.1016/j.marpolbul.2016.02.071

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n = 12), sea turtles (n = 8), and mammals (n = 3) found alive or dead in southern coast of Brazil. Enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(S), tet(M), tet(L), mrsC, and erm(B) genes by PCR. Enterococcus faecalis and Enterococcus faecium were the most common species. Single (37.09%), double (25.80%), and multiple (16.12%) antibiotic resistance patterns were observed. Resistance to rifampicin occurred most frequently. The msrC, tet(M), and/or tet(L) genes were detected in 60.15%, 73.07%, and 23.07% of the resistant strains, respectively. In conclusion, the presence of antibiotic resistant strains in these species could be related to food web interactions and aquatic pollutants or linked to environmental resistome. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：51 / 57

页数：7

共 47 条

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