High Throughput Screening of Drug Multi Residues in Fish by Quadrupole-Time-of-Flight Mass Spectrometry

A high. throughput method for screening drug multi residues was developed by quadrupole. time. of. flight(Q-TOF) mass spectrometry together with QuEChERS sample preparation technique. It has been used for the determination of 57 drugs, such as tetracyclines (TCs), sulfonamides(SAs), quinolones (QNs), triphenylmethanes (TPMs), estrogens (ETs), androgens (AGs) and glucocorticoids (GCs) in fish. The optimized pretreatment conditions were examined. The target compounds were extracted with acetonitrile under the condition of Na(2)EDTA. Mcllvaine buffer, and the usage of clean. up reagents was 25 mg anhydrous magnesium sulfate, 12.5 mg octadecylsilane and 6.25 mg N-Propylethylenediamine sorbent for extracted solvent of each milliliter. The positive results acquired by this high. throughput method were confirmed by liquid chromatography. tandem mass spectrometry (LC-MS/MS). The detection limit for these 59 drugs was in the range of 0.5-5.3 mu g/kg. The method is time. saving, convenient, effective and wide coverage. Its sensitivity can meet the requirement of the detection of drug residues in aquatic products.