Microfluidic Formation of Monodisperse Coacervate Organelles in Liposomes

被引：214

作者：

Deng, Nan-Nan ^{[1
]}

Huck, Wilhelm T. S. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Inst Mol & Mat, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2017年 / 56卷 / 33期

关键词：

artificial organelles; coacervates; liposomes; microfluidics; phase transitions; PROTOCELL MODEL; GIANT VESICLES; RNA; DROPLETS; MICROCOMPARTMENTATION; GRANULES; PHASES; MICRODROPLETS; COMPARTMENTS; TRANSITION;

D O I：

10.1002/anie.201703145

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Coacervates have been widely studied as model compartments in protocell research. Complex coacervates composed of disordered proteins and RNA have also been shown to play an important role in cellular processes. Herein, we report on a microfluidic strategy for constructing monodisperse coacervate droplets encapsulated within uniform unilamellar liposomes. These structures represent a bottomup approach to hierarchically structured protocells, as demonstrated by storage and release of DNA from the encapsulated coacervates as well as localized transcription.

引用

页码：9736 / 9740

页数：5

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