Integrating Bulk Transcriptome and Single-Cell RNA Sequencing Data Reveals the Landscape of the Immune Microenvironment in Thoracic Aortic Aneurysms

被引:15
作者
Wang, Qunhui [1 ]
Guo, Xian [1 ]
Huo, Bo [1 ]
Feng, Xin [1 ]
Fang, Ze-Min [1 ]
Jiang, Ding-Sheng [1 ,2 ,3 ,4 ]
Wei, Xiang [1 ,2 ,3 ,4 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Hosp, Sino Swiss Heart Lung Transplantat Inst, Tongji Med Coll,Div Cardiothorac & Vasc Surg, Wuhan, Peoples R China
[2] Chinese Acad Med Sci, Key Lab Organ Transplantat, Minist Educ, Wuhan, Peoples R China
[3] Chinese Acad Med Sci, NHC, Key Lab Organ Transplantat, Wuhan, Peoples R China
[4] Chinese Acad Med Sci, Key Lab Organ Transplantat, Wuhan, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
thoracic aortic aneurysm; immune infiltration; macrophages; T cells; intercellular communications; MECHANISMS; EXPRESSION; INFLAMMATION; RECRUITMENT; DISSECTION; DYNAMICS;
D O I
10.3389/fcvm.2022.846421
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Thoracic aortic aneurysm (TAA) is a life-threatening cardiovascular disease whose formation is reported to be associated with massive vascular inflammatory responses. To elucidate the roles of immune cell infiltration in the pathogenesis underlying TAA, we utilized multiple TAA datasets (microarray data and scRNA-seq data) and various immune-related algorithms (ssGSEA, CIBERSORT, and Seurat) to reveal the landscapes of the immune microenvironment in TAA. The results exhibited a significant increase in the infiltration of macrophages and T cells, which were mainly responsible for TAA formation among the immune cells. To further reveal the roles of immunocytes in TAA, we inferred the intercellular communications among the identified cells of aortic tissues. Notably, we found that in both normal aortic tissue and TAA tissue, the cells that interact most frequently are macrophages, endothelial cells (ECs), fibroblasts, and vascular smooth muscle cells (VSMCs). Among the cells, macrophages were the most prominent signal senders and receivers in TAA and normal aortic tissue. These findings suggest that macrophages play an important role in both the physiological and pathological conditions of the aorta. The present study provides a comprehensive evaluation of the immune cell composition and reveals the intercellular communication among aortic cells in human TAA tissues. These findings improve our understanding of TAA formation and progression and facilitate the development of effective medications to treat these conditions.
引用
收藏
页数:14
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