Mangiferin protects osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling

被引：35

作者：

Xia, Gen ^{[1
]}

Li, Xueru ^{[2
]}

Zhu, Xi ^{[1
]}

Yin, Xueming ^{[3
]}

Ding, Huiqiang ^{[1
]}

Qiao, Yongdong ^{[1
]}

机构：

[1] Ningxia Med Univ, Gen Hosp, Dept Orthoped, 804 South Shengli St, Yinchuan 750000, Peoples R China

[2] Ningxia Med Univ, Gen Hosp, Dept Ultrasound, Cardiovasc Dis & Cerebrovasc Dis Hosp, Yinchuan, Peoples R China

[3] Third Peoples Hosp Ningxia, Dept Gen Surg, Yinchuan, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 491卷 / 03期

关键词：

MAN; Osteoblast; Oxidative damage; ERK5; Nrf2; APOPTOTIC CELL-DEATH; EXTRACT VIMANG; STRESS; OSTEOPOROSIS; ALENDRONATE; ACTIVATION; AUTOPHAGY; DIAGNOSIS; PATHWAY; MODEL;

D O I：

10.1016/j.bbrc.2017.06.184

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress has currently been proposed as a risk factor associated with the development and proression of osteoporosis. In this study, we identify the effect of mangiferin (MAN) on apoptosis and differentiation of osteoblast-like MC3T3-E1 cells insulted by H2O2. We firstly found that MAN can promote cell proliferation of MC3T3-E1 cells in a time-and dose-dependent manner and stimulate the phosphorylation of ERK5. Cells were divided as five groups: control, H2O2 (100 mu M, control), H2O2 + MAN (5 mu M), H2O2 + MAN (10 mu M), and H2O2 + MAN (20 mu M). MAN can significantly decrease H2O2-induced apoptosis and elevated ROS level of MC3T3-E1 cells. The expressions of caspase-3, caspase-9 and Bax/Bcl-2 were increased with H2O2 treatment, and MAN can reverse these changes. In addition, Nrf2 and its downstream target effectors (Hal, NQO1) were dramatically attenuated in MC3T3-E cells treatment with H2O2, while MAN can significantly increase the expression of Nrf2, HO1 and NQO1. The expression of ERK5 was down regulated by RNA interference in MOT3-E1 cells, and we found that MAN (20 mu M) pretreatment didn't make remarkable decrease in cell apoptosis or expressions of apoptosis-related proteins in H2O2-insulted siRNA-ERK5 cells. This study indicated that MAN can protect osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling, which can be new agent for osteoporosis. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：807 / 813

页数：7

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