Metabolism of polychlorinated biphenyls by Solanum nigrum hairy root clone SNC-9O and analysis of transformation products

被引：59
作者：
Kucerová, P
Macková, M
Chromá, L
Burkhard, J
Tríska, J
Demnerová, K
Macek, T
机构：
[1] ICT Prague, Fac Food & Biochem Technol, Dept Biochem & Microbiol, Prague 16628, Czech Republic
[2] ICT Prague, Fac Environm Protect, Dept Environm Chem, Prague 16628, Czech Republic
[3] Acad Sci Czech Republic, Inst Landscape Ecol, Ceske Budejovice 37005, Czech Republic
[4] Acad Sci Czech Republic, Inst Organ Chem & Biochem, CR-16610 Prague, Czech Republic
来源：
PLANT AND SOIL | 2000年 / 225卷 / 1-2期
关键词：
environment; hairy root; phytoremediation; plant cells; polychlorinated biphenyls; Solanum nigrum; transformation products;
D O I：
10.1023/A:1026551728196
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The study investigates aspects of PCB metabolism by a hairy root culture of Solanum nigrum L. (clone SNC-9O) in vitro. Standard conditions were established for efficient, up to 72% PCB conversion (22 individual PCB congeners examined in commercial mixture Delor 103, 5 g fresh biomass in 100 ml media shaken with 5 mg PCB for 14 days). The conversion products formed from three monochlorobiphenyls were monohydroxychlorobiphenyls and dihydroxychlorobiphenyls, while six dichlorobiphenyls yielded different monohydroxydichlorobiphenyls. Efficiency of the transformation of individual PCB congeners was evaluated together with phytotoxic effect on the clone SNC-9O. Major metabolites of monochlorobiphenyls analysed after extraction from biomass were hydroxylated at the position 4, and 4', respectively.
引用
收藏
页码：109 / 115
页数：7
相关论文
共 21 条
[1]   AEROBIC AND ANAEROBIC BIODEGRADATION OF PCBS - A REVIEW [J].
ABRAMOWICZ, DA .
CRITICAL REVIEWS IN BIOTECHNOLOGY, 1990, 10 (03) :241-249
[2]   BIOREMEDIATION IN THE RHIZOSPHERE [J].
ANDERSON, TA ;
GUTHRIE, EA ;
WALTON, BT .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1993, 27 (13) :2630-2636
[3]   ANALYSIS OF POLYCHLORINATED-BIPHENYLS (PCB) BY GLASS-CAPILLARY GAS-CHROMATOGRAPHY - COMPOSITION OF TECHNICAL AROCLOR-PCB AND CLOPHEN-PCB MIXTURES [J].
BALLSCHMITER, K ;
ZELL, M .
FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1980, 302 (01) :20-31
[4]   INFLUENCE OF CHLORINE SUBSTITUTION PATTERN ON THE DEGRADATION OF POLYCHLORINATED-BIPHENYLS BY 8 BACTERIAL STRAINS [J].
BEDARD, DL ;
HABERL, ML .
MICROBIAL ECOLOGY, 1990, 20 (02) :87-102
[5]   PHYTOREMEDIATION OF CONTAMINATED SOILS [J].
CUNNINGHAM, SD ;
BERTI, WR ;
HUANG, JWW .
TRENDS IN BIOTECHNOLOGY, 1995, 13 (09) :393-397
[6]  
DEMNEROVA K, 1997, NATO ASI SER, V1, P53
[7]   METABOLISM OF 2-CHLOROBIPHENYL BY SUSPENSION-CULTURES OF PAULS SCARLET ROSE [J].
FLETCHER, JS ;
GROEGER, AW ;
MCFARLANE, JC .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1987, 39 (06) :960-965
[8]   THE INFLUENCE OF INCREASING CHLORINE CONTENT ON THE ACCUMULATION AND METABOLISM OF POLYCHLORINATED-BIPHENYLS (PCBS) BY PAULS SCARLET ROSE CELLS [J].
GROEGER, AG ;
FLETCHER, JS .
PLANT CELL REPORTS, 1988, 7 (05) :329-332
[9]   Perspectives in biodegradation of alkanes and PCBs [J].
Kas, J ;
Burkhard, J ;
Demnerova, K ;
Kostal, J ;
Macek, T ;
Mackova, M ;
Pazlarova, J .
PURE AND APPLIED CHEMISTRY, 1997, 69 (11) :2357-2369
[10]   Correlation of PCB transformation by plant tissue cultures with their morphology and peroxidase activity changes [J].
Kucerová, P ;
Macková, M ;
Poláchová, L ;
Burkhard, J ;
Demnerová, K ;
Pazlarová, J ;
Macek, T .
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1999, 64 (09) :1497-1509
123