Reflectometric detection of influenza virus in human saliva using nanoimprint lithography-based flexible two-dimensional photonic crystal biosensor

被引：68

作者：

Endo, Tatsuro ^{[1
,2
]}

Ozawa, Satoshi ^{[3
]}

Okuda, Norimichi ^{[3
]}

Yanagida, Yasuko ^{[1
,2
]}

Tanaka, Satoru ^{[3
]}

Hatsuzawa, Takeshi ^{[1
,2
]}

机构：

[1] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Mechanomicro Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[2] Tokyo Inst Technol, Precis & Intelligence Lab, Midori Ku, Yokohama, Kanagawa 2268503, Japan

[3] SCIVAX Corp, Takatsu Ku, Kawasaki, Kanagawa 2130012, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2010年 / 148卷 / 01期

关键词：

Biosensor; Nanoimprint lithography (NIL); Photonic crystal (PC); Influenza virus; Salivary monitoring; Reflectometric detection; SELF-ASSEMBLED MONOLAYERS; LABEL-FREE DETECTION;

D O I：

10.1016/j.snb.2010.05.036

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Protecting the human body from viral infections is extremely important for maintaining human health. To prevent viral infections, early diagnosis is important. Moreover, the diagnosis must also be non-invasive and non-painful. Hence, for development of novel analytical systems for protecting from viral infections, non-invasive biosensing devices with high sensitivity and high throughput are effective analytical systems that can be conveniently used to make a diagnosis. For establishment of novel analytical systems, photonic crystal (PC) can be used to observe nanometer-sized periodic structures, and their structural color can be used to realize on-site non-invasive biosensor. In this study, we have demonstrated the reflectometric detection of influenza virus in human saliva using antibody-immobilized flexible nanoimprint lithography (NIL)-based two-dimensional (2D)-PC biosensor. As a result, using antibody-immobilized 2D-PC biosensor, reflection intensity change which related with influenza virus concentrations could be observed. In addition, we could detect the influenza virions present in human saliva (detection limit: 1 ng mL(-1)). Our approach provides versatile analytical systems for the establishment of novel rapid diagnostic systems of serious viral diseases. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：269 / 276

页数：8

共 39 条

[1] High ionic strength glucose-sensing photonic crystal [J].