Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis

被引：26

作者：

Green, CB ^{[1
]}

Zhao, XM ^{[1
]}

Hoyer, LL ^{[1
]}

机构：

[1] Univ Illinois, Dept Vet Pathobiol, Urbana, IL 61801 USA

来源：

INFECTION AND IMMUNITY | 2005年 / 73卷 / 03期

关键词：

D O I：

10.1128/IAI.73.3.1852-1855.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.

引用

页码：1852 / 1855

页数：4

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