DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp strain PCC 6803

被引：135

作者：

Hihara, Y ^{[1
]}

Sonoike, K

Kanehisa, M

Ikeuchi, M

机构：

[1] Saitama Univ, Dept Biochem & Mol Biol, Fac Sci, Saitama, Saitama 3388570, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan

[3] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

[4] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Tokyo 1538902, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 05期

关键词：

D O I：

10.1128/JB.185.5.1719-1725.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

引用

页码：1719 / 1725

页数：7

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