A shorter enrichment culture for the isolation of Escherichia coli O157:H7 from raw minced beef

被引：0

作者：

Vernozy-Rozand, C ^{[1
]}

Mazuy, C ^{[1
]}

Ray-Gueniot, S ^{[1
]}

Boutrand-Loei, S ^{[1
]}

Meyrand, A ^{[1
]}

Richard, Y ^{[1
]}

机构：

[1] Ecole Natl Vet, Unite Microbiol Alimentaire Microbiol Previsionel, F-69280 Marcy Letoile, France

来源：

REVUE DE MEDECINE VETERINAIRE | 1997年 / 148卷 / 11期

关键词：

E-coli O157 : H7; isolation; meat; immunomagnetic separation;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The use of immunomagnetic separation (IMS) techniques has been reported to reduce the total test time and improve the sensitivity of microbiological tests done on foods. This study demonstrates the ability of the Dynabeads(TM) immunomagnetic separation system, to enable detection of a few cells of E. coli O157:H7 in 25g of inoculated raw minced beef after enrichment in modified tryptone soy broth (MTSB) incubated at 42 degrees C for only 4 hours. Compared to CHROMagar E. coli O157, sorbitol Mac-Conkey agar supplemented with cefixime and tellurite (CT-SMAC) was shown to be a better subculture medium.

引用

页码：879 / 882

页数：4

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