In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron 'Fragrantissimum Improved'

被引：20

作者：

Hebert, Cary J. ^{[1
]}

Touchell, Darren H. ^{[1
]}

Ranney, Thomas G. ^{[1
]}

LeBude, Anthony V. ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Hort Sci, Mt Hort Crops Res & Extens Ctr, Mills River, NC 28759 USA

来源：

HORTSCIENCE | 2010年 / 45卷 / 05期

关键词：

polyploidy; shoot organogenesis; oryzalin; thidiazuron; 6-(gamma; gamma-dimethylallylamino); purine; indole-3-acetic acid; 1-naphthaleneacetic acid; LEAF EXPLANTS; REPRODUCTIVE-BEHAVIOR; TISSUE-CULTURE; ORYZALIN; COLCHICINE; THIDIAZURON; EVERGREEN; HYBRIDS; AZALEA; LEAVES;

D O I：

10.21273/HORTSCI.45.5.801

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Rhododendron L. 'Fragrantissimum Improved' is an attractive cultivar with showy, fragrant flowers but has limited potential for breeding because it is a sterile wide hybrid. Protocols for in vitro regeneration and polyploid induction were developed for this cultivar as a means to potentially restore fertility and enhance ornamental traits. Combinations of thidiazuron (TDZ) at 0, 5, 10, 15, or 20 mu M and 1-naphthaleneacetic acid (NAA) at 0, 2.5, 5, or 10 mu M were used to induce shoot regeneration from leaves. Shoot regeneration was optimized (68% of leaf segments produced shoots) using 8.8 mu M TDZ and 10 mu M NAA. To induce polyploidy, regenerative callus was treated with 7.5, 15, 30, 60, or 90 mu M of the mitotic inhibitor oryzalin for 1, 3, 5, 7, or 14 d in various combinations. Oryzalin significantly affected survival and shoot regenerative capacity. A percentage of homogenous, tetraploid shoots was recovered from treatments of 30 mu M oryzalin for 1 (13%) or 3 (13%) days and 7.5 mu M oryzalin for 7 (20%) or 14 (7%) days.

引用

页码：801 / 804

页数：4

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