A simple and rapid spectrophotometric method for the determination of nitrite by its decolorizing effect on peroxovanadate complex

被引：25
作者：
Galil, Mansour S. Abdul [1 ]
Mahadevaiah [1 ]
Kumar, M. S. Yogendra [1 ]
Nagendrappa, G. [1 ]
机构：
[1] Univ Mysore, Dept Studies Chem, Mysore 570006, Karnataka, India
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2007年 / 67卷 / 01期
关键词：
nitrite determination; peroxovanadate; nitrite in water; soil; spectrophotometry;
D O I：
10.1016/j.saa.2006.06.026
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A rapid, simple, and most economical spectrophotometric method was proposed for the determination of nitrite in various water samples, soil samples, and roots of leguminous plants. The method is based on decolorizing effect of nitrite on complex formed between hydrogen peroxide and vanadate in acidic medium. The decolorization of that complex by nitrite was exploited to monitor the reaction spectrophotometrically at 470 nm. The method was optimized for effect of concentrations of ammonium metavanadate, hydrogen peroxide, various acids, concentrations of sulphuric acid, order of reagents addition and color stability. The color of the complex was found to be stable for about 2 days, and the stability constant of the complex was also calculated by modified Job's method. The linearity range of the calibration graph was over 6.67-66.7 mu g ml(-1) of nitrite with molar absorptivity, 0.276 x 10(3) mol(-1) 1 cm(-1) and Sandell's sensitivity, 0. 1667 mu g cm(-2). The method was applied successfully for the determination of nitrite in soil samples, various wastewater samples and roots of leguminous plants. (c) 2006 Elsevier B.V. All rights reserved.
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页码：76 / 82
页数：7
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