Distinct effects of struvite and biochar amendment on the class 1 integron antibiotic resistance gene cassettes in phyllosphere and rhizosphere

被引:47
作者
An, Xin-Li [1 ,2 ]
Chen, Qing-Lin [1 ,2 ]
Zhu, Dong [1 ,2 ]
Su, Jian-Qiang [1 ]
机构
[1] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 361021, Peoples R China
[2] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
关键词
Antibiotic resistance genes; Environmental pollution; Horizontal gene transfer; Land application; Soil remediation; SOIL; ABUNDANCE; BACTERIA; DIVERSITY; ELEMENTS; VEGETABLES; FERTILIZER; COMMUNITY; IMPACT; HARBOR;
D O I
10.1016/j.scitotenv.2018.03.034
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Struvite recovered from wastewater is promising for recycling phosphorus into soil as fertilizers. However, struvite application may prompt the proliferation of antibiotic resistance in soil and plant. This study examined the impacts of struvite application and biochar amendment on integrons abundance and gene cassette contexts in rhizosphere soil and phyllosphere using quantitative PCR and clone library analysis. Microcosm experiments revealed that class 1 integron was the most prevalent in all samples, with higher concentration and higher relative abundance in rhizosphere than those in phyllosphere. The majority of resistance gene cassettes were associated with genes encoding resistance to aminoglycosides, beta-lactams and chloramphenicols. Struvite application significantly increased the genetic diversity of antibiotic resistance gene cassettes in both rhizosphere and phyllosphere. However, biochar amendment attenuated the increasing effect of struvite application exerting on the class 1 integron antibiotic resistance gene cassette pool in phyllosphere. These findings highlighted human activities to be the source of integron gene cassette pool and raised the possibility of using biochar amendment as an alternative mean for mitigating antibiotic resistance in environments. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:668 / 676
页数:9
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