A New Water-Soluble Nanomicelle Formed through Self-Assembly of Pectin-Curcumin Conjugates: Preparation, Characterization, and Anticancer Activity Evaluation

被引：66
作者：
Bai, Feng [1 ,2 ]
Diao, Jiajing [1 ]
Wang, Ying [1 ,2 ]
Sun, Shixin [1 ]
Zhang, Hongmei [1 ]
Liu, Yunyun [1 ]
Wang, Yanqing [1 ]
Cao, Jian [1 ]
机构：
[1] Yancheng Teachers Univ, Inst Appl Chem & Environm Engn, Yancheng City 224002, Jiangsu, Peoples R China
[2] Nanjing Univ Technol, Chem & Chem Engn, Nanjing 210009, Jiangsu, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2017年 / 65卷 / 32期
基金：
中国国家自然科学基金;
关键词：
curcumin; pectin; nanomicelles; antioxidant activity; cytotoxicity; IN-VITRO; BIOMEDICAL APPLICATIONS; ANTIOXIDANT ACTIVITY; CHEMICAL-STABILITY; AQUEOUS SOLUBILITY; PROSTATE-CANCER; NANOPARTICLES; CELLS; PH; POLYSACCHARIDE;
D O I：
10.1021/acs.jafc.7b02250
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Curcumin is a dominating active component of Curcuma longa and has been studied widely because of its prominent biological activities. The extremely low aqueous solubility, stability, and bioavailability of curcumin limit its application in the field of medicine. In this study, we developed pectin-curcumin (PEC-CCM) conjugates that could self-assemble water-soluble nanomicelles in aqueous solution. The structure of PEC-CCM conjugates was characterized by ultraviolet-visible spectra, fluorescence spectra, Fourier transform infrared spectroscopy, and H-1 nuclear magnetic resonance spectroscopy. The thermal property of PEC-CCM conjugates was investigated by thermogravimetric analysis. It was found that PEC-CCM conjugates had formed nanomicelles in aqueous medium via self-assembly. These nanomicelles were observed as small spheres or ellipsoids and aggregated with a size range of 70-190 nm by transmission electron microscopy analysis. In a solution of nanomicelles, the stability of curcumin was improved, and its antioxidant property was preserved. The anticancer activity of PEC-CCM conjugates was quantified by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,S-diphenyltetrazolium bromide] assay using a hepatic cancer cell line (HepG2), a breast cancer cell line (MCF-7), a cervical cancer cell line (HeLa), and a human normal kidney cell line (293A). It was found that the curcumin of PEC-CCM conjugates had a more significant inhibitory effect on cancer cells and was less cytotoxic to normal cells than free curcumin was. PEC-CCM conjugates have great potential for some food and pharmaceutical applications.
引用
收藏
页码：6840 / 6847
页数：8
相关论文
共 43 条
[1]   Synthesis and characterization of pectin derivative with antitumor property against Caco-2 colon cancer cells [J].
Almeida, Elizangela A. M. S. ;
Facchi, Suelen P. ;
Martins, Alessandro F. ;
Nocchi, Samara ;
Schuquel, Ivania T. A. ;
Nakamura, Celso V. ;
Rubira, Adley F. ;
Muniz, Edvani C. .
CARBOHYDRATE POLYMERS, 2015, 115 :139-145
[2]   Nanoparticle assisted solvent selective transdermal combination therapy of curcumin and 5-flurouracil for efficient cancer treatment [J].
Anirudhan, T. S. ;
Nair, Anoop S. ;
Bino, Sabari J. .
CARBOHYDRATE POLYMERS, 2017, 173 :131-142
[3]   Development and characterization of anti-inflammatory activity of curcumin-loaded biodegradable microspheres with potential use in intestinal inflammatory disorders [J].
Blanco-Garcia, E. ;
Otero-Espinar, F. J. ;
Blanco-Mendez, J. ;
Leiro-Vidal, J. M. ;
Luzardo-Alvarez, A. .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2017, 518 (1-2) :86-104
[4]   Biocompatible Lipid Nanoparticles as Carriers To Improve Curcumin Efficacy in Ovarian Cancer Treatment [J].
Bondi, Maria Luisa ;
Emma, Maria Rita ;
Botto, Chiara ;
Augello, Giuseppa ;
Azzolina, Antonina ;
Di Gaudio, Francesca ;
Craparo, Emanuela Fabiola ;
Cavallaro, Gennara ;
Bachvarov, Dimcho ;
Cervello, Melchiorre .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65 (07) :1342-1352
[5]   Soy Soluble Polysaccharide as a Nanocarrier for Curcumin [J].
Chen, Fei-Ping ;
Ou, Shi-Yi ;
Chen, Zhong ;
Tang, Chuan-He .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65 (08) :1707-1714
[6]   Core-Shell Soy Protein-Soy Polysaccharide Complex (Nano)particles as Carriers for Improved Stability and Sustained Release of Curcumin [J].
Chen, Fei-Ping ;
Ou, Shi-Yi ;
Tang, Chuan-He .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (24) :5053-5059
[7]   Nanocomplexation between Curcumin and Soy Protein Isolate: Influence on Curcumin Stability/Bioaccessibility and in Vitro Protein Digestibility [J].
Chen, Fei-Ping ;
Li, Bian-Sheng ;
Tang, Chuan-He .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2015, 63 (13) :3559-3569
[8]   Selective guanosine binding and cytotoxicity of a benzimidazole derived dinickel complex [J].
Chen, Zhanfen ;
Wang, Xiaoyong ;
Zhu, Yangguang ;
Li, Ylzhi ;
Guo, Zijlan .
JOURNAL OF INORGANIC BIOCHEMISTRY, 2007, 101 (11-12) :1894-1902
[9]   Conjugation of curcumin onto alginate enhances aqueous solubility and stability of curcumin [J].
Dey, Soma ;
Sreenivasan, K. .
CARBOHYDRATE POLYMERS, 2014, 99 :499-507
[10]   Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata [J].
Duan, XJ ;
Zhang, WW ;
Li, XM ;
Wang, BG .
FOOD CHEMISTRY, 2006, 95 (01) :37-43
12345