Lithocholic acid upregulates uPAR and cell invasiveness via MAPK and AP-1 signaling in colon cancer cells

被引：45

作者：

Baek, Min Kyung ^{[1
]}

Park, Jung Sun ^{[1
]}

Park, Ji Hye ^{[1
]}

Kim, Mi Ha ^{[1
]}

Kim, Ho Dong ^{[1
]}

Bae, Woo Kyun ^{[1
]}

Chung, Ik Joo ^{[1
]}

Shin, Boo Ahn ^{[1
]}

Do Jung, Young ^{[1
]}

机构：

[1] Chonnam Natl Univ, Sch Med, Dept Biochem, Ctr Biomed Human Resources,Brain Korea Project 21, Kwangju 501190, South Korea

来源：

CANCER LETTERS | 2010年 / 290卷 / 01期

关键词：

LCA; Erk-1/2; AP-1; uPAR; Colon cancer; PLASMINOGEN-ACTIVATOR SYSTEM; UROKINASE RECEPTOR; PROTEIN-KINASE; GROWTH-FACTOR; BILE-ACIDS; INACTIVATION; PROLIFERATION; METASTASIS; INHIBITION; MIGRATION;

D O I：

10.1016/j.canlet.2009.08.030

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The secondary bile acid lithocholic acid (LCA) induced expression of urokinase-type plasminogen activator receptor (uPAR) and enhanced cell invasiveness in colon cancer cells. A dominant negative mutant or a specific inhibitor of MEK-1 suppressed LCA-induced uPAR expression. Deletions and site-directed mutagenesis revealed that the AP-1 site was required for LCA-induced uPAR transcription. LCA-mediated enhanced cell invasiveness was partially abrogated by uPAR neutralizing antibody and inhibitors of both Erk-1/2 and AP-1. These results suggest that LCA induces uPAR expression via Erk-1/2 and AP-1 pathway and, in turn, stimulate invasiveness of human colon cancer cells. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：123 / 128

页数：6

共 22 条

[1] Inactivation of uPA and Its Receptor uPAR by 3,3′-Diindolylmethane (DIM) Leads to the Inhibition of Prostate Cancer Cell Growth and Migration [J].