Liquid chromatography-electrospray ionization-tandem mass spectrometric assay for d-aspartate N-methyltransferase activity in ark shells

被引：1
作者：
Shibata, Kimihiko [1 ,2 ]
Hagiya, Saho [1 ]
Okawara, Rena [1 ]
Abe, Katsumasa [2 ]
Takahashi, Shouji [2 ]
Kera, Yoshio [2 ]
机构：
[1] Fukushima Coll, Natl Inst Technol KOSEN, Dept Appl Chem & Biochem, Iwaki, Fukushima, Japan
[2] Nagaoka Univ Technol, Dept Bioengn, Nagaoka, Niigata, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2020年 / 84卷 / 03期
关键词：
N-methyl-D-aspartate; NMDA synthase; D-Aspartate N-methyltransferase; LC-ESI-MS; MS; ark shell; METHYL-D-ASPARTATE; AMINO-ACIDS; BLOOD SHELL; SCAPHARCA-BROUGHTONII; RACEMASE; DERIVATIVES; BIVALVES; TISSUES; MUSCLE;
D O I：
10.1080/09168451.2019.1689094
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the separation and quantification of the enantiomers of N-methylaspartate and N-methylglutamate, after derivatization with N-alpha-(5-fluoro-2,4-dinitrophenyl)-L-leucinamide was established. The time required for the LC-ESI-MS/MS analysis was within 20 min and the detection limit was approximately 10 fmol per injection, demonstrating that this method can be used for the rapid determination of D-aspartate N-methyltransferase activity in the ark shell clam Scapharca broughtonii.
引用
收藏
页码：500 / 506
页数：7
相关论文
共 25 条
[1]   Cloning and expression of the pyridoxal 5′-phosphate-dependent aspartate racemase gene from the bivalve mollusk Scapharca broughtonii and characterization of the recombinant enzyme [J].
Abe, K ;
Takahashi, S ;
Muroki, Y ;
Kera, Y ;
Yamada, RH .
JOURNAL OF BIOCHEMISTRY, 2006, 139 (02) :235-244
[2]   Occurrence of D-aspartic acid and N-methyl-D-aspartic acid in rat neuroendocrine tissues and their role in the modulation of luteinizing hormone and growth hormone release [J].
D'Aniello, A ;
Di Fiore, MM ;
Fisher, GH ;
Milone, A ;
Seleni, A ;
D'Aniello, S ;
Perna, AF ;
Ingrosso, D .
FASEB JOURNAL, 2000, 14 (05) :699-714
[3]   Occurrence and neuroendocrine role of D-aspartic acid and N-methyl-D-aspartic acid in Ciona intestinalis [J].
D'Aniello, A ;
Spinelli, P ;
De Simone, A ;
D'Aniello, S ;
Branno, M ;
Aniello, F ;
Fisher, GH ;
Di Fiore, MM ;
Rastogi, RK .
FEBS LETTERS, 2003, 552 (2-3) :193-198
[4]   N-methyl-D-aspartic acid (NMDA) in the nervous system of the amphioxus Branchiostoma lanceolatum [J].
D'Aniello, Salvatore ;
Fisher, George H. ;
Topo, Enza ;
Ferrandino, Gabriele ;
Garcia-Fernandez, Jordi ;
D'Aniello, Antimo .
BMC NEUROSCIENCE, 2007, 8 (1)
[5]   Chiral separation of N-methyl-dl-aspartic acid in rat brain tissue as N-ethoxycarbonylated (S)-(+)-2-octyl ester derivatives by GC-MS [J].
Duc-Toan Nguyen ;
Kim, Kyoung-Rae ;
Lee, Gwang ;
Paik, Man-Jeong .
BIOMEDICAL CHROMATOGRAPHY, 2012, 26 (11) :1353-1356
[6]   Quantitative determination of free D-Asp, L-Asp and N-methyl-D-aspartate in mouse brain tissues by chiral separation and Multiple Reaction Monitoring tandem mass spectrometry [J].
Fontanarosa, Carolina ;
Pane, Francesca ;
Sepe, Nunzio ;
Pinto, Gabriella ;
Trifuoggi, Marco ;
Squillace, Marta ;
Errico, Francesco ;
Usiello, Alessandro ;
Pucci, Piero ;
Amoresano, Angela .
PLOS ONE, 2017, 12 (06)
[7]   A nonempirical method using LC/MS for determination of the absolute configuration of constituent amino acids in a peptide: Combination of Marfey's method with mass spectrometry and its practical application [J].
Fujii, K ;
Ikai, Y ;
Oka, H ;
Suzuki, M ;
Harada, K .
ANALYTICAL CHEMISTRY, 1997, 69 (24) :5146-5151
[8]   Sea shell diversity and rapidly evolving secretomes: insights into the evolution of biomineralization [J].
Kocot, Kevin M. ;
Aguilera, Felipe ;
McDougall, Carmel ;
Jackson, Daniel J. ;
Degnan, Bernard M. .
FRONTIERS IN ZOOLOGY, 2016, 13
[9]   Enantioselective two-dimensional high-performance liquid chromatographic determination of N-methyl-D-aspartic acid and its analogues in mammals and bivalves [J].
Koga, Reiko ;
Miyoshi, Yurika ;
Negishi, Eiichi ;
Kaneko, Tsuneaki ;
Mita, Masashi ;
Lindner, Wolfgang ;
Hamase, Kenji .
JOURNAL OF CHROMATOGRAPHY A, 2012, 1269 :255-261
[10]   EYES AS OPTICAL ALARM SYSTEMS IN FAN WORMS AND ARK CLAMS [J].
NILSSON, DE .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1994, 346 (1316) :195-212
123