Non-enzymatic electrochemical biosensor based on Pt NPs/RGO-CS-Fc nano-hybrids for the detection of hydrogen peroxide in living cells

被引：117

作者：

Bai, Zhihao ^{[1
]}

Li, Guiyin ^{[1
,3
]}

Liang, Jingtao ^{[1
]}

Su, Jing ^{[3
]}

Zhang, Yue ^{[2
]}

Chen, Huaizhou ^{[2
]}

Huang, Yong ^{[1
,3
]}

Sui, Weiguo ^{[2
]}

Zhao, Yongxiang ^{[3
]}

机构：

[1] Guilin Univ Elect Technol, Sch Life & Environm Sci, Guilin 541004, Guangxi, Peoples R China

[2] Guilin 181st Hosp, Guangxi Key Lab Metab Dis Res, Guilin 541002, Guangxi, Peoples R China

[3] Collaborat Innovat Ctr Targeting Tumor Diag & The, Natl Ctr Int Res Biol Targeting Diag & Therapy, Guangxi Key Lab Biol Targeting Diag & Therapy Res, Nanning 530021, Guangxi, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 82卷

基金：

中国国家自然科学基金;

关键词：

Non-enzymatic sensor; Graphene; Hydrogen peroxide; Ferrocene carboxylic acid; Platinum nanoparticles; NITROGEN-DOPED GRAPHENE; OXIDATIVE STRESS; H2O2; GLUCOSE; SENSOR; GOLD; AMPLIFICATION; REDUCTION; FERROCENE; FE3O4;

D O I：

10.1016/j.bios.2016.04.004

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A highly sensitive non-enzymatic electrochemical sensor based on platinum nanoparticles/reduced graphene oxide-chitosan-ferrocene carboxylic acid nano-hybrids (Pt NPs/RGO-CS-Fc biosensor) was developed for the measurement of hydrogen peroxide (H2O2). The RGO-CS-Fc nano-hybrids was prepared and characterized by UV-vis spectrum, Fourier transform infrared spectroscopy, transmission electron microscopy, Raman spectrometer and electrochemical impedance spectroscopy. Under optimal experimental conditions, the Pt NPs/RGO-CS-Fc biosensor showed outstanding catalytic activity toward H2O2 reduction. The current response of the biosensor presented a linear relationship with H2O2 concentration from 2.0 x 10(-8) M to 3.0 x 10(-8) M with a correlation coefficient of R-2=0.9968 and with logarithm of H2O2 concentration from 6.0 x 10(-6) M to 1.0 x 10(-2) M with a correlation coefficient of R-2=0.9887, the low detection limit of 20 nM was obtained at the signal/noise (SAN) ratio of 3. Moreover, the Pt NPs/RGO-CS-Fc biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2. The biosensor was also successfully applied for the detection of H2O2 from living cells containing normal and cancer cells. All these results prove that the Pt NPs/RGO-CS-Fc biosensor has the potential application in clinical diagnostics to evaluate oxidative stress of different living cells. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：185 / 194

页数：10

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