Obstacles and advances in non-viral gene delivery

被引：0

作者：

Lollo, CP ^{[1
]}

Banaszczyk, MG ^{[1
]}

Chiou, HC ^{[1
]}

机构：

[1] Immune Response Corp, Carlsbad, CA 92008 USA

来源：

CURRENT OPINION IN MOLECULAR THERAPEUTICS | 2000年 / 2卷 / 02期

关键词：

cytoplasmic entry; gene-delivery; non-viral; particle stabilization; targeting;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This review focuses on recent progress and novel strategies to improve the efficiency of in vivo non-viral gene delivery. Examples of the most promising attempts to overcome specific barriers are presented in fuller detail. Current research into several of the most difficult steps in the gene delivery pathway is discussed including particle stabilization, targeting, cytoplasmic entry and access to the nucleus. The impact of recent reports on our current understanding of the true limitations to in vivo delivery is also discussed. The importance of preclinical animal models fbr the development of clinical applications of gene therapy is noted.

引用

页码：136 / 142

页数：7

共 54 条

[1] Metabolic stability of glutaraldehyde cross-linked peptide DNA condensates [J].