Fluid shear stress induces cell migration via RhoA-YAP1-autophagy pathway in liver cancer stem cells

被引：10
|
作者：
Yan, Zhiping [1 ,2 ,3 ,4 ]
Guo, Danfeng [1 ,2 ,3 ,4 ]
Tao, Ruolin [1 ,2 ,3 ,4 ]
Yu, Xiao [1 ,2 ,3 ,4 ]
Zhang, Jiacheng [1 ,2 ,3 ,4 ]
He, Yuting [1 ,2 ,3 ,4 ]
Zhang, Jiakai [1 ,2 ,3 ,4 ]
Li, Jie [1 ,2 ,3 ,4 ]
Zhang, Shuijun [1 ,2 ,3 ,4 ]
Guo, Wenzhi [1 ,2 ,3 ,4 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, Zhengzhou 450052, Peoples R China
[2] Henan Liver Transplantat Ctr, Zhengzhou 450052, Henan, Peoples R China
[3] Henan Res Ctr Organ Transplantat, Zhengzhou 450052, Peoples R China
[4] Henan Key Lab Digest Organ Transplantat, Zhengzhou 450052, Peoples R China
来源：
CELL ADHESION & MIGRATION | 2022年 / 16卷 / 01期
基金：
中国博士后科学基金;
关键词：
Fluid shear stress; autophagy; LCSCs; migration;
D O I：
10.1080/19336918.2022.2103925
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Fluid shear stress (FSS) regulates the metastasis of hepatocellular carcinoma (HCC), but the role of the RhoA-YAP1-autophagy pathway in HCC remains unclear. Due to the core role of liver cancer stem cells (LCSCs) in HCC metastasis and recurrence, we explored the RhoA-YAP1-autophagy pathway in LCSCs under FSS. Our results indicate that LCSCs have stronger proliferation and cell spheroidization abilities. FSS (1 dyn/cm(2)) upregulated the migration of LCSCs and autophagy protein markers, inducing LC3B aggregation and autophagosome formation in LCSCs. Mechanistically, FSS promoted YAP1 dephosphorylation and transport to the nucleus, which is mediated by RhoA, inducing autophagy. Finally, inhibition of autophagy suppressed cell migration in LCSCs under FSS. In conclusion, FSS promoted the migration of LCSCs via the RhoA-YAP1-autophagy pathway.
引用
收藏
页码：94 / 106
页数：13
相关论文
共 50 条
  • [1] Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells
    Yan, Zhiping
    Su, Guanyue
    Gao, Wenbo
    He, Jia
    Shen, Yang
    Zeng, Ye
    Liu, Xiaoheng
    CELL ADHESION & MIGRATION, 2019, 13 (01) : 152 - 162
  • [2] Low-level shear stress induces differentiation of liver cancer stem cells via the Wnt/β-catenin signalling pathway
    Sun, Jinghui
    Luo, Qing
    Liu, Lingling
    Song, Guanbin
    EXPERIMENTAL CELL RESEARCH, 2019, 375 (01) : 90 - 96
  • [3] Low-level shear stress promotes migration of liver cancer stem cells via the FAK-ERK1/2 signalling pathway
    Sun, Jinghui
    Luo, Qing
    Liu, Lingling
    Song, Guanbin
    CANCER LETTERS, 2018, 427 : 1 - 8
  • [4] The Interaction of Adrenomedullin and Macrophages Induces Ovarian Cancer Cell Migration via Activation of RhoA Signaling Pathway
    Pang, Xiaoyan
    Shang, Hai
    Deng, Boya
    Wen, Fang
    Zhang, Yi
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (02): : 2774 - 2787
  • [5] Fluid shear stress activates YAP1 to promote cancer cell motility
    Lee, Hyun Jung
    Diaz, Miguel F.
    Price, Katherine M.
    Ozuna, Joyce A.
    Zhang, Songlin
    Sevick-Muraca, Eva M.
    Hagan, John P.
    Wenzel, Pamela L.
    NATURE COMMUNICATIONS, 2017, 8
  • [6] Fluid shear stress activates YAP1 to promote cancer cell motility
    Hyun Jung Lee
    Miguel F. Diaz
    Katherine M. Price
    Joyce A. Ozuna
    Songlin Zhang
    Eva M. Sevick-Muraca
    John P. Hagan
    Pamela L. Wenzel
    Nature Communications, 8
  • [7] NESFATIN-1 INDUCES CELL MIGRATION VIA AN AUTOCRINE PATHWAY IN PROSTATE CANCER CELLS
    Yoo, TagKeun
    Cho, HeeJu
    Im, Eunji
    Yang, Hyunwon
    INTERNATIONAL JOURNAL OF UROLOGY, 2014, 21 : A228 - A228
  • [8] Nanofibers Regulate Single Bone Marrow Stem Cell Osteogenesis via FAK/RhoA/YAP1 Pathway
    Chang, Bei
    Ma, Chi
    Liu, Xiaohua
    ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (39) : 33022 - 33031
  • [9] YAP promotes gastric cancer cell survival and migration/invasion via the ERK/endoplasmic reticulum stress pathway
    Liu, Haibin
    Mei, Dong
    Xu, Pengcheng
    Wang, Haisheng
    Wang, Yan
    ONCOLOGY LETTERS, 2019, 18 (06) : 6752 - 6758
  • [10] Urotensin II Induces Migration of Endothelial Progenitor Cells via Activation of the RhoA/Rho Kinase Pathway
    Xu, Shengkai
    Jiang, Hong
    Wu, Bing
    Yang, Jian
    Chen, Sisi
    TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2009, 219 (04): : 283 - 288
12345