Metabolomics: Approaches to assessing oocyte and embryo quality

被引:97
作者
Singh, R. [1 ]
Sinclair, K. D. [1 ]
机构
[1] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England
关键词
metabolomics; oocyte quality; embryo quality; metabolites; mass spectroscopy;
D O I
10.1016/j.theriogenology.2007.04.007
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Morphological evaluation remains the primary method of embryo assessment during IVF cycles, but its modest predictive power and inherent inter- and intra-observer variability limits its value. Low-molecular weight metabolites represent the end products of cell regulatory processes and therefore reveal the response of biological systems to a variety of genetic, nutrient or environmental influences. It follows that the non-invasive quantification of oocyte and embryo metabolism, from the analyses of follicular fluid or culture media, may be a useful predictor of pregnancy outcome following embryo transfer, a potential supported by recent clinical studies working with specific classes of metabolites such as glycolytic intermediates and amino acids. Such selective approaches, however, whilst adhering closely to known cellular processes, may fail to harness the full potential of contemporary metabolomic methodologies, which can measure a wider spectrum of metabolites. However, an important technical drawback with many existing methodologies is the limited number of metabolites that can be determined by a single analytical platform. Vibrational spectroscopy methodologies such as Fourier transform infrared and near infrared spectroscopy may overcome these limitations by generating unique spectral signatures of functional groups and bonds, but their application in embryo quality assessment remains to be fully validated. Ultimately, a combination of evaluation criteria that include morphometry with metabolomics may provide the best predictive assessment of embryo viability. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:S56 / S62
页数:7
相关论文
共 33 条
[1]  
AGRAWAL A, 2006, P 2 INT C CRYOPR HUM
[2]  
Aharoni Asaph, 2002, OMICS A Journal of Integrative Biology, V6, P217, DOI 10.1089/15362310260256882
[3]  
Alikani M, 2002, ASSESSMENT OF MAMMALIAN EMBRYO QUALITY: INVASIVE AND NON-INVASIVE TECHNIQUES, P1
[4]   Network biology:: Understanding the cell's functional organization [J].
Barabási, AL ;
Oltvai, ZN .
NATURE REVIEWS GENETICS, 2004, 5 (02) :101-U15
[5]   Amino acid depletion and appearance during porcine preimplantation embryo development in vitro [J].
Booth, PJ ;
Humpherson, PG ;
Watson, TJ ;
Leese, HJ .
REPRODUCTION, 2005, 130 (05) :655-668
[6]   Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover [J].
Brison, DR ;
Houghton, FD ;
Falconer, D ;
Roberts, SA ;
Hawkhead, J ;
Humpherson, PG ;
Lieberman, BA ;
Leese, HJ .
HUMAN REPRODUCTION, 2004, 19 (10) :2319-2324
[7]   Metabolomics: Current analytical platforms and methodologies [J].
Dunn, WB ;
Ellis, DI .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2005, 24 (04) :285-294
[8]   Metabolomics by numbers: acquiring and understanding global metabolite data [J].
Goodacre, R ;
Vaidyanathan, S ;
Dunn, WB ;
Harrigan, GG ;
Kell, DB .
TRENDS IN BIOTECHNOLOGY, 2004, 22 (05) :245-252
[10]   Correlation between protein and mRNA abundance in yeast [J].
Gygi, SP ;
Rochon, Y ;
Franza, BR ;
Aebersold, R .
MOLECULAR AND CELLULAR BIOLOGY, 1999, 19 (03) :1720-1730