MicroRNA-125b inhibits lens epithelial cell apoptosis by targeting p53 in age-related cataract

被引：65

作者：

Qin, Yu ^{[1
]}

Zhao, Jiangyue ^{[1
]}

Min, Xiaojie ^{[1
]}

Wang, Mingwu ^{[2
]}

Luo, Wenting ^{[3
]}

Wu, Di ^{[1
]}

Yan, Qichang ^{[1
]}

Li, Jing ^{[1
]}

Wu, Xinwei ^{[1
]}

Zhang, Jinsong ^{[1
]}

机构：

[1] Fourth Affiliated Hosp China Med Univ, Eye Hosp China Med Univ, Dept Ophthalmol, Key Lens Res Lab Liaoning Prov, Shenyang 110005, Liaoning, Peoples R China

[2] Univ Arizona, Coll Med, Dept Ophthalmol & Vis Sci, Tucson, AZ USA

[3] China Med Univ, Shengjing Hosp, Key Lab Hlth Minist Congenital Malformat, Liaoning, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2014年 / 1842卷 / 12期

基金：

中国国家自然科学基金;

关键词：

microRNA-125b; p53; Apoptosis; Gene expression regulation; Cataract; VISUAL IMPAIRMENT; TUMOR-SUPPRESSOR; EXPRESSION; CANCER; PREVALENCE; MEDIATORS; EXPOSURE; PRIMATES; LIVER; MIRNA;

D O I：

10.1016/j.bbadis.2014.10.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNA-125b (miR-125b) has been implicated in a variety of diseases as either repressors or promoters, and plays crucial roles in many cellular processes such as cell differentiation, proliferation and apoptosis. Age-related cataract has become one of the most serious problems facing the aging population in the world. The purpose of this study was to investigate the role of miR-125b in the development of age-related cataract. We demonstrated that miR-125b was downregulated in both age-related cataract tissue and lens epithelial cell apoptosis induced by UV irradiation. We also identified the impact of miR-125b on apoptosis in a lens epithelial cell line. In vitro, miR-125b regulates human lens epithelial cell apoptosis at least in part by directly targeting p53. In addition, an inverse relationship between miR-125b and p53 expression was seen in age-related cataract tissue. In conclusion, this study suggests that miR-125b might be closely involved in the pathogenesis of cataract, and has the potential to be a diagnostic biomarker or even a therapeutic modality for cataract. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：2439 / 2447

页数：9

共 44 条

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