Isolation and characterization of the Serratia entomophila antifeeding prophage

被引：57

作者：

Hurst, Mark R. H. ^{[1
]}

Beard, Sam S. ^{[1
]}

Jackson, Trevor A. ^{[1
]}

Jones, Sandra M. ^{[1
]}

机构：

[1] Cantebury Agr & Sci Ctr, AgRes, Biocontrol & Biosecur, Lincoln, New Zealand

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 270卷 / 01期

关键词：

antifeeding; afp; RfaH; Serratia entomophila; Costelytra zealandica; PVC;

D O I：

10.1111/j.1574-6968.2007.00645.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Serratia entomophila antifeeding prophage (Afp) is thought to form a virus-like structure that has activity towards the New Zealand grass grub, Costelytra zealandica. Through the trans based expression of AnfA1, an RfaH - like transcriptional antiterminator, the Afp, was able to be induced. The expressed Afp was purified and visualized by electron microscopy. The Afp resembled a phage tail-like bacteriocin, exhibiting two distinct morphologies: an extended and a contracted form. The purified Afp conferred rapid activity towards C. zealandica larvae, causing cessation of feeding and a change to an amber colouration within 48 h postinoculation, with increased dose rates causing larval mortality.

引用

页码：42 / 48

页数：7

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