Mis-Spliced Transcripts of Nicotinic Acetylcholine Receptor α6 Are Associated with Field Evolved Spinosad Resistance in Plutella xylostella (L.)

被引:115
作者
Baxter, Simon W. [1 ]
Chen, Mao [2 ]
Dawson, Anna [1 ]
Zhao, Jian-Zhou [2 ]
Vogel, Heiko [3 ]
Shelton, Anthony M. [2 ]
Heckel, David G. [3 ]
Jiggins, Chris D. [1 ]
机构
[1] Univ Cambridge, Dept Zool, Cambridge, England
[2] Cornell Univ, Dept Entomol, New York State Agr Expt Stn, Ithaca, NY 14853 USA
[3] Max Planck Inst Chem Ecol, Dept Entomol, Jena, Germany
基金
澳大利亚研究理事会; 英国生物技术与生命科学研究理事会;
关键词
DIAMONDBACK MOTH LEPIDOPTERA; RED FLOUR BEETLE; BACILLUS-THURINGIENSIS; GENE FAMILY; HELIOTHIS-VIRESCENS; TOBACCO BUDWORM; MUSCA-DOMESTICA; POPULATION; NOCTUIDAE; DIVERSITY;
D O I
10.1371/journal.pgen.1000802
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The evolution of insecticide resistance is a global constraint to agricultural production. Spinosad is a new, low-environmental-risk insecticide that primarily targets nicotinic acetylcholine receptors (nAChR) and is effective against a wide range of pest species. However, after only a few years of application, field evolved resistance emerged in the diamondback moth, Plutella xylostella, an important pest of brassica crops worldwide. Spinosad resistance in a Hawaiian population results from a single incompletely recessive and autosomal gene, and here we use AFLP linkage mapping to identify the chromosome controlling resistance in a backcross family. Recombinational mapping with more than 700 backcross progeny positioned a putative spinosad target, nAChR alpha 6 (Px alpha 6), at the resistance locus, PxSpinR. A mutation within the ninth intron splice junction of Px alpha 6 results in mis-splicing of transcripts, which produce a predicted protein truncated between the third and fourth transmembrane domains. Additional resistance-associated Pxa6 transcripts that excluded the mutation containing exon were detected, and these were also predicted to produce truncated proteins. Identification of the locus of resistance in this important crop pest will facilitate field monitoring of the spread of resistance and offer insights into the genetic basis of spinosad resistance in other species.
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页数:10
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