Comparison of DOTA and NODAGA as chelates for 68Ga-labelled CDP1 as novel infection PET imaging agents

被引：5
|
作者：
Mdlophane, A. H. [1 ,2 ,3 ]
Ebenhan, T. [1 ,2 ,4 ]
Marjanovic-Painter, B. [3 ]
Govender, T. [5 ,6 ]
Sathekge, M. M. [1 ,2 ,4 ]
Zeevaart, J. R. [3 ,4 ,7 ]
机构：
[1] Univ Pretoria, Nucl Med, Crn Malherbe & Steve Biko Rd, ZA-0001 Pretoria, South Africa
[2] Steve Biko Acad Hosp, Crn Malherbe & Steve Biko Rd, ZA-0001 Pretoria, South Africa
[3] South African Nucl Energy Corp Necsa, Radiochem, Pelindaba, Bldg P1600, ZA-0240 Brits, South Africa
[4] Pelindaba, Preclin Imaging Facil, Nucl Med Res Infrastruct NuMeRI, ZA-0240 Brits, South Africa
[5] Univ KwaZulu Natal, Sch Hlth Sci, Catalysis & Peptide Res Unit, ZA-4001 Durban, South Africa
[6] Univ KwaZulu Natal, Sch Chem & Phys, ZA-4001 Durban, South Africa
[7] North West Univ, Dept Sci & Technol Preclin Drug Dev Platform, 11 Hoffman St, ZA-2520 Potchefstroom, South Africa
来源：
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2019年 / 322卷 / 02期
关键词：
CDP1; NODAGA; DOTA; Ga-68; Antimicrobial peptides; Infection imaging; PET; ANTIMICROBIAL PEPTIDES; UBIQUICIDIN; ANTIBACTERIAL; COMPLEXES; TRACER; GA-68; LL-37;
D O I：
10.1007/s10967-019-06693-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The cathelicidin-derived peptide (CDP1) is a human antimicrobial peptide that preferentially targets bacterial membranes in response to infection. CDP1 was functionalised with NODAGA and DOTA for complexation with gallium-68 to evaluate its potential as an infection imaging tracer. The synthesis of [Ga-68]Ga-NODAGA-CDP1 and [Ga-68]Ga-DOTA-CDP1 were optimised for pH, molarity, incubation time and temperature, and product purification. The integrity and protein binding were investigated employing [Ga-68]GaCl3 and [Ga-68]Ga-DOTA-TATE as internal references. [Ga-68]Ga-NODAGA-CDP1 displayed good labelling properties with higher product yield compared to [Ga-68]Ga-DOTA-CDP1. In contrast, [Ga-68]Ga-DOTA-CDP1 showed better stability and is the preferred candidate for an in vivo investigation.
引用
收藏
页码：629 / 638
页数：10
相关论文
共 44 条
  • [1] Comparison of DOTA and NODAGA as chelates for 68Ga-labelled CDP1 as novel infection PET imaging agents
    A. H. Mdlophane
    T. Ebenhan
    B. Marjanovic-Painter
    T. Govender
    M. M. Sathekge
    J. R. Zeevaart
    Journal of Radioanalytical and Nuclear Chemistry, 2019, 322 : 629 - 638
  • [2] Synthesis, in vitro evaluation, and 68Ga-radiolabeling of CDP1 toward PET/CT imaging of bacterial infection
    Dutta, Jyotibon
    Baijnath, Sooraj
    Somboro, Anou M.
    Nagiah, Savania
    Albericio, Fernando
    de la Torre, Beatriz G.
    Marjanovic-Painter, Biljana
    Zeevaart, Jan Rijn
    Sathekge, Mike
    Kruger, Hendrik G.
    Chuturgoon, Anil
    Naicker, Tricia
    Ebenhan, Thomas
    Govender, Thavendran
    CHEMICAL BIOLOGY & DRUG DESIGN, 2017, 90 (04) : 572 - 579
  • [3] In vivo comparison of DOTA based 68Ga-labelled bisphosphonates for bone imaging in non-tumour models
    Meckel, Marian
    Fellner, Marco
    Thieme, Natalie
    Bergmann, Ralf
    Kubicek, Vojteck
    Roesch, Frank
    NUCLEAR MEDICINE AND BIOLOGY, 2013, 40 (06) : 823 - 830
  • [4] PET imaging of PARP expression using 68Ga-labelled inhibitors
    Wang, Xiangwei
    Liu, Wei
    Li, Ke
    Chen, Kaiwen
    He, Simin
    Zhang, Jianping
    Gu, Bingxin
    Xu, Xiaoping
    Song, Shaoli
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2023, 50 (09) : 2606 - 2620
  • [5] 68Ga-labelled desferrioxamine-B for bacterial infection imaging
    Petrik, Milos
    Umlaufova, Eva
    Raclavsky, Vladislav
    Palyzova, Andrea
    Havlicek, Vladimir
    Pfister, Joachim
    Mair, Christian
    Novy, Zbynek
    Popper, Miroslav
    Hajduch, Marian
    Decristoforo, Clemens
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2021, 48 (02) : 372 - 382
  • [6] 68Ga-labelled desferrioxamine-B for bacterial infection imaging
    Milos Petrik
    Eva Umlaufova
    Vladislav Raclavsky
    Andrea Palyzova
    Vladimir Havlicek
    Joachim Pfister
    Christian Mair
    Zbynek Novy
    Miroslav Popper
    Marian Hajduch
    Clemens Decristoforo
    European Journal of Nuclear Medicine and Molecular Imaging, 2021, 48 : 372 - 382
  • [7] Preparation of 68Ga-labelled DOTA-peptides using a manual labelling approach for small-animal PET imaging
    Romero, Eduardo
    Martinez, Alfonso
    Oteo, Marta
    Garcia, Angel
    Angel Morcillo, Miguel
    APPLIED RADIATION AND ISOTOPES, 2016, 107 : 113 - 120
  • [8] Hybrid Imaging of Aspergillus fumigatus Pulmonary Infection with Fluorescent, 68Ga-Labelled Siderophores
    Pfister, Joachim
    Summer, Dominik
    Petrik, Milos
    Khoylou, Marta
    Lichius, Alexander
    Kaeopookum, Piriya
    Kochinke, Laurin
    Orasch, Thomas
    Haas, Hubertus
    Decristoforo, Clemens
    BIOMOLECULES, 2020, 10 (02)
  • [9] A comparison of three 67/68Ga-labelled exendin-4 derivatives for β-cell imaging on the GLP-1 receptor: the influence of the conjugation site of NODAGA as chelator
    Jodal, Andreas
    Lankat-Buttgereit, Brigitte
    Brom, Maarten
    Schibli, Roger
    Behe, Martin
    EJNMMI RESEARCH, 2014, 4 : 1 - 10
  • [10] A comparison of three 67/68Ga-labelled exendin-4 derivatives for β-cell imaging on the GLP-1 receptor: the influence of the conjugation site of NODAGA as chelator
    Andreas Jodal
    Brigitte Lankat-Buttgereit
    Maarten Brom
    Roger Schibli
    Martin Béhé
    EJNMMI Research, 4
12345