Identification of MicroRNAs Involved in Resistance to Sunitinib in Renal Cell Carcinoma Cells

被引：45

作者：

Yamaguchi, Noriya ^{[1
,2
]}

Osaki, Mitsuhiko ^{[1
,3
]}

Onuma, Kunishige ^{[1
]}

Yumioka, Tetsuya ^{[2
]}

Iwamoto, Hideto ^{[2
]}

Sejima, Takehiro ^{[2
]}

Kugoh, Hiroyuki ^{[3
,4
]}

Takenaka, Atsushi ^{[2
]}

Okada, Futoshi ^{[1
,3
]}

机构：

[1] Tottori Univ, Div Pathol Biochem, Tottori, Japan

[2] Tottori Univ, Dept Surg, Div Urol, Fac Med, Tottori, Japan

[3] Tottori Univ, Chromosome Engn Res Ctr, Tottori, Japan

[4] Tottori Univ, Dept Biomed Sci, Inst Regenerat Med & Biofunct, Grad Sch Med Sci, Tottori, Japan

来源：

ANTICANCER RESEARCH | 2017年 / 37卷 / 06期

关键词：

miRNA; renal cell carcinoma; sunitinib; tyrosine kinase inhibitor; SEQUESTRATION; METASTASIS; LYSOSOMES;

D O I：

10.21873/anticanres.11652

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aim: To generate sunitinib-resistant renal cell carcinoma (RCC) cell lines and identify miRNAs contributing to sunitinib resistance. Materials and Methods: Two RCC cell lines, ACHN and RCC23, were cultured by continuous treatment with sunitinib for 3 months, with doses gradually increasing up to the 50% inhibitory concentration for each cell line. We performed microarray and quantitative real-time polymerase chain reaction analyses of sunitinib-resistant ACHN (SR-ACHN) and RCC23 (SR-RCC23) cells, as well of as sunitinib-sensitive ACHN and RCC23 cells. Results: SR-ACHN and SR-RCC23 cells exhibited significantly higher resistance to sunitinib treatment compared to sunitinib-sensitive cells. SR-ACHN and SR-RCC23 cells were hypertrophic and contained granules in the cytoplasm. When SR-ACHN and SR-RCC23 cells were compared to ACHN and RCC23 cells, expression of miR-575, miR-642b-3p, and miR-4430 was significantly increased, while that of miR-18a-5p, miR-29b-1-5p, miR-431-3p, and miR-4521 was significantly decreased. Conclusion: These miRNAs may contribute to sunitinib resistance in humans.

引用

页码：2985 / 2992

页数：8

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