Background suppression by axially selective activation in single-molecule localization microscopy

被引：2
作者：
Chen, Danni [1 ,2 ]
Yu, Bin [1 ]
Qu, Junle [1 ]
Niu, Hanben [1 ]
机构：
[1] Shenzhen Univ, Key Lab Optoelect Devices & Syst, Minist Educ Guangdong Prov, Inst Optoelect, Shenzhen 518060, Peoples R China
[2] Huazhong Univ Sci & Technol, Coll Optoelect Sci & Engn, Wuhan 430074, Peoples R China
来源：
OPTICS LETTERS | 2010年 / 35卷 / 06期
基金：
中国国家自然科学基金;
关键词：
FLUORESCENCE MICROSCOPY; DIFFRACTION-LIMIT; RESOLUTION; SAMPLES; STORM;
D O I：
10.1364/OL.35.000886
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Resolution of a few tens of nanometers has been achieved in fluorescence microscopy with photoswitchable molecules. However, for thick samples, the background brought by the crosstalk of unwanted on-state molecules is nonnegligible. Now we present a background suppression method by using two axial standing waves generated by the interference of two activation beams with the same phases and two deactivation beams with the opposite phases. With spatially incoherent illumination, most activated molecules are located in a thin layer. The performance of such method is simulated with the known photoswitching characteristic of Cy5. With suitable parameters, the thickness of the layer can reach 39 nm (FWHM). (C) 2010 Optical Society of America
引用
收藏
页码：886 / 888
页数：3
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