Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

被引：34

作者：

Hoyos-Mallecot, Y. ^{[1
,2
]}

Riazzo, C. ^{[1
]}

Miranda-Casas, C. ^{[1
]}

Rojo-Martin, M. D. ^{[1
]}

Gutierrez-Fernandez, J. ^{[1
,2
]}

Navarro-Mari, J. M. ^{[1
]}

机构：

[1] Univ Hosp Virgen Nieves, Dept Microbiol, Granada 18014, Spain

[2] Univ Granada, Postgrad Programme, Granada, Spain

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 105卷

关键词：

MALDI-TOF; Carbapenemases; Blood culture; DESORPTION-IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; METALLO-BETA-LACTAMASES; ENTEROBACTERIACEAE; RESISTANCE; INSTRUMENT; BACTEREMIA; ASSAY;

D O I：

10.1016/j.mimet.2014.07.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5 h with high sensitivity and specificity. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：98 / 101

页数：4

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