Promotion of skin carcinogenesis by dimethylarsinic acid in keratin (K6)/ODC transgenic mice

被引:40
作者
Morikawa, T [1 ]
Wanibuchi, H [1 ]
Morimura, K [1 ]
Ogawa, M [1 ]
Fukushima, S [1 ]
机构
[1] Osaka City Univ, Sch Med, Dept Pathol 1, Abeno Ku, Osaka 5458585, Japan
来源
JAPANESE JOURNAL OF CANCER RESEARCH | 2000年 / 91卷 / 06期
关键词
K6/ODC transgenic mice; ODC; DMA; skin carcinogenesis;
D O I
10.1111/j.1349-7006.2000.tb00984.x
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Dimethylarsinic acid (DMA) is a major metabolite of inorganic arsenicals in mammals, and arsenic exposure is associated with tumor development in a wide variety of human tissues, particularly the skin. Transgenic mice with ornithine decarboxylase (ODC) targeted to hair follicle keratinocytes are much more sensitive than littermate controls to carcinogens. In this study we investigated the promoting effect of DR DMA on skin carcinogenesis in such K6/0DC transgenic mice. The back skin of female C57BL/6J k6/0DC transgenic mice, 10 to 14 weeks old, was initiated with topical application of 7,12-dimethylbenz[a]anthracene (DMBA) at a dose of 50 mu g or acetone alone on day 1 of the experiment, followed by treatment with 3.6 mg of DMA, 5 mu g of 12-O-tetradecanoylphorbol-13-acetate (TPA) or neutral: vehicle (control) twice a week for 18 weeks. Mice were killed 1 week after the end of the treatment. Induction of skin tumors was significantly accelerated in the DMA-treated group, as well as in the TPA-treated group, indicating that DMA has a promoting effect on skin tumorigenesis in K6/0DC transgenic mice.
引用
收藏
页码:579 / 581
页数:3
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