Clinical significance of miRNA-autophagy transcript expression in patients with hepatocellular carcinoma

被引：2

作者：

Matboli, Marwa ^{[1
]}

Shafei, Ayman E. ^{[2
]}

Shehata, Hanan H. ^{[1
]}

Nabil, Nesreen ^{[3
]}

Hossam, Nourhan ^{[1
]}

Azazy, Ahmed E. M. ^{[1
,4
]}

El-Tawdi, Ahmed H. F. ^{[5
]}

Abdel-Rahman, Omar ^{[6
]}

机构：

[1] Ain Shams Univ, Med Biochem & Mol Biol Dept, Oncol Diagnost Unit, Fac Med, POB 11381, Cairo, Egypt

[2] Mil Armed Forces Coll Med, Biomed Res Dept, Cairo, Egypt

[3] Modern Univ Technol & Informat, Fac Pharm, Dept Biochem, Cairo, Egypt

[4] Armed Forces Coll Med, Cairo, Egypt

[5] Mil Med Acad, Gen & Plast Surg Dept, Cairo, Egypt

[6] Ain Shams Univ, Clin Oncol Dept, Fac Med, Cairo, Egypt

来源：

BIOMARKERS IN MEDICINE | 2017年 / 11卷 / 08期

关键词：

bioinformatics; biomarkers; C-JUN; hepatocellular carcinoma; LAMP2; miRNA; prognosis; HEPATITIS; MICRORNA; PROGNOSIS; LAMP-2; BIOMARKERS; APOPTOSIS; MIR-146A;

D O I：

10.2217/bmm-2017-0049

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aim: This study integrates autophagy transcripts miRNAs expression based on bioinformatic analysis followed by clinical validation. Methodology: Cellular jun proto-oncogene mRNA, LAMP2 mRNA, miR-16 and miR-146a level were investigated in the serum and tissue of patients with hepatocellular carcinoma (HCC), chronic hepatitis C and healthy volunteers by quantitative real-time PCR. The prognostic power of this serum RNA panel was explored. Results: The expression of serum cellular jun proto-oncogene mRNA, LAMP2 mRNA, miR-16 and miR-146a were positive in 85.1, 94, 97.1 and 84.2% HCC patients, respectively and they were correlated with tissue levels. Our results suggested that the chosen panel is an independent prognostic factor for survival in patients with HCC. Conclusion: The current work provides four RNA-based biomarker panel for HCC diagnosis and prognosis.

引用

页码：641 / 655

页数：15

共 44 条

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