Incorporation of osteopontin peptide into kidney stone-related calcium oxalate monohydrate crystals: a quantitative study

被引:13
作者
Gleberzon, Jared S. [1 ,2 ]
Liao, Yinyin [2 ]
Mittler, Silvia [3 ,4 ]
Goldberg, Harvey A. [1 ,2 ]
Grohe, Bernd [2 ,4 ]
机构
[1] Univ Western Ontario, Schulich Sch Med & Dent, Dept Biochem, London, ON N6A 5C1, Canada
[2] Univ Western Ontario, Schulich Sch Med & Dent, Sch Dent, London, ON N6A 5C1, Canada
[3] Univ Western Ontario, Dept Phys & Astron, London, ON N6A 3K7, Canada
[4] Univ Western Ontario, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
Calcium oxalate monohydrate; Osteopontin peptides; Phosphorylation; Quantitative peptide adsorption; Quantitative peptide incorporation; Kidney stone-related crystal growth; INTRACRYSTALLINE PROTEINS; ORGANIC MATRIX; SEX-HORMONES; URINARY; GROWTH; INHIBITION; CRYSTALLIZATION; WHEWELLITE; ADSORPTION; MECHANISM;
D O I
10.1007/s00240-018-01105-x
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Polyelectrolyte-crystal interactions regulate many aspects of biomineralization, including the shape, phase, and aggregation of crystals. Here, we quantitatively investigate the role of phosphorylation in interactions with calcium oxalate monohydrate crystals (COM), using synthetic peptides corresponding to the sequence 220-235 in osteopontin, a major inhibitor of kidney stone-related COM formation. COM formation is induced in the absence or presence of fluorescent-labeled peptides containing either no (P0), one (P1) or three (P3) phosphates and their adsorption to and incorporation into crystals determined using quantitative fluorimetry (also to determine maximum adsorption/incorporation), confocal/scanning electron microscopy and X-ray/Raman spectroscopy. Results demonstrate that higher phosphorylated peptides show stronger irreversible adsorption to COM crystals (P3: K-0 similar to 66.4 x 10(6) M-1; P1: K-0 similar to 29.4 x 10(6) M-1) and higher rates of peptide incorporation into crystals (maximum: P3: similar to 58.8 ng and P1: similar to 8.9 ng per mu g of COM) than peptides containing less phosphate groups. However, crystals grown at that level of incorporable P3 show crystal-cleavage. Therefore, extrapolation of maximum incorporable P3 was carried out for crystals that are still intact, resulting in similar to 49.1 ng P3 mu g(-1) COM (or similar to 4.70 wt%). Both processes, adsorption and incorporation, proceed via the crystal faces {100} > {121} > {010} (from strongest to weakest), with X-ray and Raman spectroscopy indicating no significant effect on the crystal structure. This suggests a process in which the peptide is surrounded by growing crystal matrix and then incorporated. In general, knowing the quantity of impurities in crystalline/ceramic matrices (e.g., kidney stones) provides more control over stress/strain or solubilities, and helps to categorize such composites.
引用
收藏
页码:425 / 440
页数:16
相关论文
共 86 条
[81]   Control of calcium oxalate crystal structure and cell adherence by urinary macromolecules [J].
Wesson, JA ;
Worcester, EM ;
Wiessner, JH ;
Mandel, NS ;
Kleinman, JG .
KIDNEY INTERNATIONAL, 1998, 53 (04) :952-957
[82]   HYDROXYAPATITE MINERALIZATION AND DEMINERALIZATION IN THE PRESENCE OF SYNTHETIC PHOSPHORYLATED PENTAPEPTIDES [J].
WIKIEL, K ;
BURKE, EM ;
PERICH, JW ;
REYNOLDS, EC ;
NANCOLLAS, GH .
ARCHIVES OF ORAL BIOLOGY, 1994, 39 (08) :715-721
[83]   Interfacial free energies and crystallization in aqueous media [J].
Wu, WJ ;
Nancollas, GH .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 182 (02) :365-373
[84]   Hydroxyapatite Growth Inhibition Effect of Pellicle Statherin Peptides [J].
Xiao, Y. ;
Karttunen, M. ;
Jalkanen, J. ;
Mussi, M. C. M. ;
Liao, Y. ;
Grohe, B. ;
Lagugne-Labarthet, F. ;
Siqueira, W. L. .
JOURNAL OF DENTAL RESEARCH, 2015, 94 (08) :1106-1112
[85]   The influence of sex hormones on renal osteopontin expression and urinary constituents in experimental urolithiasis [J].
Yagisawa, T ;
Ito, F ;
Osaka, Y ;
Amano, H ;
Kobayashi, C ;
Toma, H .
JOURNAL OF UROLOGY, 2001, 166 (03) :1078-1082
[86]   POSTMENOPAUSAL HORMONE AND THE RISK OF NEPHROLITHIASIS: A META-ANALYSIS [J].
Yu, Juan ;
Yin, Binyan .
EXCLI JOURNAL, 2017, 16 :986-994