A two-color acid-free cartilage and bone stain for zebrafish larvae

被引：566
作者：
Walker, M. B.
Kimmel, C. B.
机构：
[1] Stowers Inst Med Res, Kansas City, MO USA
[2] Univ Oregon, Eugene, OR 97403 USA
来源：
BIOTECHNIC & HISTOCHEMISTRY | 2007年 / 82卷 / 01期
关键词：
alcian blue; alizarin red; PCR genotyping;
D O I：
10.1080/10520290701333558
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.
引用
收藏
页码：23 / 28
页数：6
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