Non-covalent decoration of carbon dots with folic acid via a polymer-assisted strategy for fast and targeted cancer cell fluorescence imaging

被引：50

作者：

Lei, Dan ^{[1
]}

Yang, Wen ^{[1
]}

Gong, Yunqian ^{[1
]}

Jing, Jing ^{[1
]}

Nie, Hailiang ^{[1
]}

Yu, Bin ^{[1
]}

Zhang, Xiaoling ^{[1
]}

机构：

[1] Beijing Inst Technol, Sch Chem, Beijing Key Lab Photoelect Electrophoton Convers, Key Lab Cluster Sci,Minist Educ, Beijing 100081, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 230卷

基金：

高等学校博士学科点专项科研基金;

关键词：

Non-covalent decoration; Carbon dots; Folate acid; Folate receptor-positive cancer cells; Turn on fluorescent nanoprobe; GRAPHENE QUANTUM DOTS; IN-VIVO; FOLATE; RECEPTOR; NANOPARTICLES; PHOTOLUMINESCENCE; DIAGNOSTICS;

D O I：

10.1016/j.snb.2016.02.123

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An efficient approach for targeting and detecting folate-receptor (FR)-positive cancer cells was developed through non-covalent conjugation of folic acid (FA) on polyethyleneimine modified CDs (PEI-CDs). The fluorescent CDs were prepared by a facile hydrothermal method, and their surfaces were wrapped with positively charged PEI for further conjugation with FA through electrostatic interaction. The FA targeted PEI modified CDs (FA-PEI-CDs) can be used as a turn-on fluorescent nanoprobe for folate receptor (FR) positive cancer cells in vivo and in vitro. The uptake of the designed FA-PEI-CDs by HeLa and HepG2 cancer cells was verified by confocal laser scanning microscopy after 10 min incubation and competition experiments, as well as a comparative study with FR-negative MCF-7 cells. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：714 / 720

页数：7

共 48 条

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