Isolation of the first toxin from the scorpion Buthus occitanus israelis showing preference for Shaker potassium channels

被引：18
|
作者：
Kominsky-Atias, Adi
Somech, Erez
Zilberberg, Noam [1 ]
机构：
[1] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Zlotowski Ctr Neurosci, IL-84105 Beer Sheva, Israel
来源：
FEBS LETTERS | 2007年 / 581卷 / 13期
基金：
以色列科学基金会;
关键词：
alpha-KTx3; potassium channels; scorpion toxin; shaker; TEVC; buthus occitanus;
D O I：
10.1016/j.febslet.2007.04.065
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have purified BoiTx1, the first toxin from the venom of the Israeli scorpion, Buthus occitanus israelis, and studied its activity and genomic organization. BoiTx1 is a 37 amino acid-long peptide contained six conserved cysteines, and is classified as an alpha-KTx3.10 toxin. The pharmacological effects of BoiTx1 were studied on various cloned K+ channels expressed in Xenopus laevis oocytes. BoiTx1 inhibited currents through Drosophila Shaker channels with an IC50 value of 3.5 +/- 0.5 nM, yet had much lesser effect on its mammalian orthologs. Thus, BoiTx1 is the first member of the alpha-KTx3 family that preferentially affects insect potassium channels. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2478 / 2484
页数：7
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