Nano-electrospray mass spectrometry with a modified commercial IonSpray source

Electrospray mass spectrometry is a standard tool for the investigation of biological samples. Due to the high flowrates of the standard sources, large sample amounts are required and it is almost impossible to spray physiological solutions due to their aqueous medium. The introduction of microelectrospray sources has made it possible to decrease the sample amounts needed and enabled the use of buffered solutions. In this work, a nanoES-like source based on a modification of an existing IonSpray source is introduced. In contrast to other nanoES sources available, the modification presented allows a fast change between the nanoES and the normal IonSpray modes. Copyright (C) 2000 John Wiley & Sons, Ltd.